Aqp5^{tm1.1(cre)Pfl}
Targeted Allele Detail

Summary

• Symbol: Aqp5^{tm1.1(cre)Pfl}
• Name: aquaporin 5; targeted mutation 1.1, Per Flodby
• MGI ID: MGI:5051940
• Synonyms: ACID, Aqp5-cre, Aqp5^{tm1.1(cre,DsRed)Pfl}
• Gene: Aqp5 Location: Chr15:99488909-99492710 bp, + strand Genetic Position: Chr15, 56.13 cM
• Alliance: Aqp5^{tm1.1(cre)Pfl} page

Mutation origin

• Germline Transmission: Earliest citation of germline transmission: J:174124
• Parent Cell Line: W2 (ES Cell)
• Strain of Origin: 129S6/SvEvTac

Mutation description

• Allele Type: Targeted (Recombinase, Reporter)
• Mutations: Insertion, Intragenic deletion
• Aqp5^{tm1.1(cre)Pfl} expression driven by 1 gene
  Knock-in expression driven by:
  

  Organism	Driver Gene	Note
  mouse	Aqp5 (MGI:106215)

• Mutation details: A cre-IRES-DsRed cassette with a nuclear localization signal at the 5' end of the cre sequence (and an intronic sequence placed inside the cre gene to increase expression) was knocked into the Aqp5 locus by homologous recombination in W2 ES cells. The cre cassette was inserted into exon 1, with the endogenous start codon of Aqp5 being replaced by the ATG start codon of cre recombinase. Recombined ES cells were injected into blastocysts to produce chimeric animals, The Frt-flanked PGK-neo selection that had been inserted with the cre-IRES-DsRed cassette was subsequently excised by crossing chimeric males to Gt(ROSA)26Sor^tm1(FLP1)Dym animals. Cre activity was analyzed by crossing Aqp5-cre mice to R26lacZ or R26mT/mG reporter animals. Expression was detected in alveolar type I (AT1) cells in adult lungs. (J:174124)

Recombinase activity

Activity:	Tissue activity of this recombinase allele
Driver:	Aqp5 (mouse) Summary of all recombinase alleles driven by Aqp5. Alliance mouse Aqp5 gene page

Find Mice (IMSR)

• Mouse strains and cell lines available from the International Mouse Strain Resource (IMSR)
• Carrying this Mutation: Mouse Strains: 0 strains available Cell Lines: 0 lines available
• Carrying any Aqp5 Mutation: 19 strains or lines available

References

• Original: J:174124 Flodby P, et al., Directed expression of Cre in alveolar epithelial type 1 cells. Am J Respir Cell Mol Biol. 2010 Aug;43(2):173-8
• All: 21 reference(s)