Tg(Pdgfra-cre/ERT)467Dbe
Transgene Detail

Summary

• Symbol: Tg(Pdgfra-cre/ERT)467Dbe
• Name: transgene insertion 467, Dwight E Bergles
• MGI ID: MGI:5056164
• Synonyms: PDGRaR-CreER
• Transgene: Tg(Pdgfra-cre/ERT)467Dbe Location: unknown
• Alliance: Tg(Pdgfra-cre/ERT)467Dbe page

Transgene origin

• Strain of Origin: C57BL/6 x SJL

Transgene description

• Transgene Type: Transgenic (Inducible, Recombinase)
• Inducer: tamoxifen
• Mutation: Insertion
• Tg(Pdgfra-cre/ERT)467Dbe expression driven by 1 gene
  Transgene expression driven by:
  

  Organism	Driver Gene	Note
  mouse	Pdgfra (MGI:97530)

• Mutation details: A BAC clone (RP24-148N) was modified by homologous recombination to generate an inducible cre transgene. The cDNA encoding tamoxifen-inducible cre recombinase (cre/ERT) was inserted after the 5' UTR, followed by a rabbit beta-globin poly adenylation sequence. The linearized 150kb construct was injected into pronuclei of 1- or 2-cell stage nuclei from B6SJL mice. Four founders were obtained and crossed to Z/EG reporter animals to assay EGFP expression in offspring. Line 467 displayed the highest cre efficiency and was chosen for further studies. (J:167748)

Recombinase activity

Activity:	Tissue activity of this recombinase allele
Driver:	Pdgfra (mouse) Summary of all recombinase alleles driven by Pdgfra. Comparative matrix view of recombinase activities Alliance mouse Pdgfra gene page

Find Mice (IMSR)

• Mouse strains and cell lines available from the International Mouse Strain Resource (IMSR)
• Carrying this Mutation: Mouse Strains: 1 strain available Cell Lines: 0 lines available

References

• Original: J:167748 Kang SH, et al., NG2+ CNS glial progenitors remain committed to the oligodendrocyte lineage in postnatal life and following neurodegeneration. Neuron. 2010 Nov 18;68(4):668-81
• All: 131 reference(s)