H2-T3^{tm2.1(CAG-EGFP)Maoh}
Targeted Allele Detail

Summary

• Symbol: H2-T3^{tm2.1(CAG-EGFP)Maoh}
• Name: histocompatibility 2, T region locus 3; targeted mutation 2.1, Masato Ohtsuka
• MGI ID: MGI:5056530
• Gene: H2-T3 Location: Chr17:36496463-36501043 bp, - strand Genetic Position: Chr17, 18.99 cM
• Alliance: H2-T3^{tm2.1(CAG-EGFP)Maoh} page

Mutation origin

• Germline Transmission: Earliest citation of germline transmission: J:173808
• Parent Cell Line: E14TG2a (ES Cell)
• Strain of Origin: 129P2/OlaHsd

Mutation description

• Allele Type: Targeted (Conditional ready, Null/knockout, RMCE-ready, Reporter)
• Mutations: Insertion, Intragenic deletion
• Mutation details: Recombinase-mediated cassette exchange (RMCE) replaced the expression cassette between the JT15 and lox2272 sites of H2-T3^tm1Maoh with (5' to 3') an IRES, lacZ, polyadenylation sequence, CAG promoter, hygro gene, polyadenylation sequence, FRT site, polyadenylation sequence, and CAG driven EGFP. Flp-mediated recombination removes all but the CAG promoter EGFP reporter construct flanked by an FRT site and the lox2272 site. (J:173808)

Find Mice (IMSR)

• Mouse strains and cell lines available from the International Mouse Strain Resource (IMSR)
• Carrying this Mutation: Mouse Strains: 1 strain available Cell Lines: 0 lines available
• Carrying any H2-T3 Mutation: 25 strains or lines available

References

• Original: J:173808 Ohtsuka M, et al., Pronuclear injection-based mouse targeted transgenesis for reproducible and highly efficient transgene expression. Nucleic Acids Res. 2010 Dec 1;38(22):e198
• All: 1 reference(s)