Dnajc30^{tm1(KOMP)Vlcg}
Targeted Allele Detail

Summary

• Symbol: Dnajc30^{tm1(KOMP)Vlcg}
• Name: DnaJ heat shock protein family (Hsp40) member C30; targeted mutation 1, Velocigene
• MGI ID: MGI:5085552
• Synonyms: Dnajc30^-
• Gene: Dnajc30 Location: Chr5:135093060-135094219 bp, + strand Genetic Position: Chr5, 74.97 cM
• Alliance: Dnajc30^{tm1(KOMP)Vlcg} page
• IMPC: Dnajc30 gene page

Mutation origin

• Mutant Cell Lines: 16795A-A9, 16795A-F1, 16795A-H2
• Germline Transmission: Earliest citation of germline transmission: J:188991
• Parent Cell Line: VGB6 (ES Cell)
• Strain of Origin: C57BL/6NTac
• Project Collection: KOMP-Regeneron

Mutation description

• Allele Type: Targeted (Null/knockout, Reporter)
• Mutations: Insertion, Intragenic deletion Vector: ZEN-Ub1
• Mutation details: The insertion of Velocigene cassette ZEN-Ub1 created a deletion of size 749bp between positions 135093108-135093856 of Chromosome 5 (Build GRCm39). Droplet digital PCR (ddPCR) analysis confirmed that Dnajc30 expression was severely reduced in homozygous mutant mice. (J:136110, J:266469)

Expression

In Structures Affected by this Mutation:

4 anatomical structure(s)

Find Mice (IMSR)

• Mouse strains and cell lines available from the International Mouse Strain Resource (IMSR)
• Carrying this Mutation: Mouse Strains: 1 strain available Cell Lines: 1 line available
• Carrying any Dnajc30 Mutation: 11 strains or lines available

Notes

Cell lines 16795A-A9 and 16795A-F1 were successfully used to make chimeric mice. Germline transmission was accomplished. J:266469

Two independent lines of knockout mice were created from ES cell clones 16795A-F1 (line 1) and 16795A-A9 (line 2). Both lines exhibited similar patterns of expression and gross phenotypes. J:266469 focused primarily on line 2 generated from clone 16795A-A9.

References

• Original: J:136110 Velocigene, Alleles produced for the KOMP project by Velocigene (Regeneron Pharmaceuticals). MGI Direct Data Submission. 2008
• All: 3 reference(s)