Tg(Eno2-Glrb)456Wha
Transgene Detail

Summary

• Symbol: Tg(Eno2-Glrb)456Wha
• Name: transgene insertion 456, Hans Weiher
• MGI ID: MGI:5086014
• Synonyms: rat GlyR beta transgene, TG456, Tg(NSE-Glrb)456
• Transgene: Tg(Eno2-Glrb)456Wha Location: unknown
• Alliance: Tg(Eno2-Glrb)456Wha page

Transgene origin

• Strain of Origin: (C57BL/6 x DBA/2)F1 x DBA/2

Transgene description

• Transgene Type: Transgenic (Inserted expressed sequence)
• Mutation: Insertion
• Tg(Eno2-Glrb)456Wha expresses 1 gene
  Transgene expresses:
  

  Organism

  Expressed Gene

  Homolog in Mouse

  Note

  rat

  Glrb (25456)

  Glrb	(MGI:95751)

• Mutation details: The transgene contains the promoter of the rat enolase 2, gamma neuronal gene driving expression of a cDNA encoding the beta subunit of the rat glycine receptor, which is followed by the SV40 small T-antigen splice and polyadenylation signals. . Brains of both heterozygous and homozygous transgenic mice of line 456 on a homozygous Glrb^spa background contain Glrb mRNA and membrane-bound glycine receptor alpha-1 subunit (a proxy for the beta receptor subunit), analyzed via mRNA northern and western blot analysis, respectively, at levels not significantly different from those in non-transgenic Glrb^spa homozygotes. Despite this, binding of strychnine to spinal cord membrane preparations from the homozygous transgenic mice is consistently higher than to preps from the non-transgenic animals, though still quite low. (J:32212, J:60037)
• Inheritance: Recessive

Find Mice (IMSR)

• Mouse strains and cell lines available from the International Mouse Strain Resource (IMSR)
• Carrying this Mutation: Mouse Strains: 0 strains available Cell Lines: 0 lines available

Notes

This is one of four transgenic founder lines that were derived (J:32212). Despite the very low expression of the transgene, when homozygous it rescues the reproductive and much of the neurologic phenotype of Glrb^spa/ Glrb^spa mice (J:60037).

References

• Original: J:32212 Hartenstein B, et al., Low level expression of glycine receptor beta subunit transgene is sufficient for phenotype correction in spastic mice. EMBO J. 1996 Mar 15;15(6):1275-82
• All: 2 reference(s)