Tg(Thy1-GLRA1)783Wha
Transgene Detail

Summary

• Symbol: Tg(Thy1-GLRA1)783Wha
• Name: transgene insertion 783, Hans Weiher
• MGI ID: MGI:5086269
• Synonyms: tg271R, tg271R-783, Tg(Thy1-Glra1)783Wha
• Transgene: Tg(Thy1-GLRA1)783Wha Location: unknown
• Alliance: Tg(Thy1-GLRA1)783Wha page

Transgene origin

• Strain of Origin: (C57BL/6 X DBA/2)F1 x C57BL/6

Transgene description

• Transgene Type: Transgenic (Humanized sequence, Inserted expressed sequence)
• Mutation: Insertion
• Mutation details: This transgene contains a cDNA encoding the human glycine receptor alpha 1 subunit. The human cDNA has replaced, in an expression vector, a segment of the mouse thymus antigen 1 gene whose deletion limits expression to neurons. RT-PCR using primers that amplify both mouse and human Glra1/GLRA1 cDNA demonstrates elevated mRNA expression in the spinal cord and forebrain of transgenic versus wild-type mice. In situ hybridization analysis confirms higher expression in transgenic brains and reveals sites of ectopic expression. While competition between endogenous and transgene-derived alpha 1 receptor subunits for endogenous beta subunits confounds quantitative ligand-binding analysis, presence of complete receptors at sites of ectopic GLRA1 mRNA expression in transgenic, but not wild-type, brains is shown by competitive and non-competitive ligand binding to frozen brain sections. (J:76009)

Expression

In Structures Affected by this Mutation:

1 anatomical structure(s)

Find Mice (IMSR)

• Mouse strains and cell lines available from the International Mouse Strain Resource (IMSR)
• Carrying this Mutation: Mouse Strains: 0 strains available Cell Lines: 0 lines available

Notes

Ten transgenic mouse lines were derived from founder mice bearing this transgenic construct; four lines, which the authors designate tg271R-731, tg271R-783, tg271R791 and tg271R803, were maintained for analysis. Line 783 is the strongest expressing. ( J:76009).

References

• Original: J:76009 Becker L, et al., Disease-specific human glycine receptor alpha1 subunit causes hyperekplexia phenotype and impaired glycine- and GABA(A)-receptor transmission in transgenic mice. J Neurosci. 2002 Apr 1;22(7):2505-12
• All: 1 reference(s)