Pcp4^tm1.1Jim
Targeted Allele Detail

Summary

• Symbol: Pcp4^tm1.1Jim
• Name: Purkinje cell protein 4; targeted mutation 1.1, James I Morgan
• MGI ID: MGI:5086270
• Gene: Pcp4 Location: Chr16:96268806-96326993 bp, + strand Genetic Position: Chr16, 56.97 cM
• Alliance: Pcp4^tm1.1Jim page

Mutation origin

• Germline Transmission: Earliest citation of germline transmission: J:174091
• Parent Cell Line: W9.5/W95 (ES Cell)
• Strain of Origin: 129S1/Sv-Oca2⁺ Tyr⁺ Kitl⁺

Mutation description

• Allele Type: Targeted (Null/knockout)
• Mutations: Insertion, Intragenic deletion
• Mutation details: A loxP site was inserted upstream of exon 3. An IRES-lacZ-neo cassette with a 5' loxP site was inserted downstream of exon 3. Cre-mediated recombination removed exon 3 and allowed for the expression of the lacZ gene. The absence of protein expression was confirmed by western blot analysis on cerebellar extracts. (J:174091)

Find Mice (IMSR)

• Mouse strains and cell lines available from the International Mouse Strain Resource (IMSR)
• Carrying this Mutation: Mouse Strains: 0 strains available Cell Lines: 0 lines available
• Carrying any Pcp4 Mutation: 7 strains or lines available

References

• Original: J:174091 Wei P, et al., Impaired Locomotor Learning and Altered Cerebellar Synaptic Plasticity in pep-19/pcp4-Null Mice. Mol Cell Biol. 2011 Jul;31(14):2838-44
• All: 2 reference(s)