Trp53^tm4Att
Targeted Allele Detail

Summary

• Symbol: Trp53^tm4Att
• Name: transformation related protein 53; targeted mutation 4, Laura D Attardi
• MGI ID: MGI:5140078
• Synonyms: p53^{LSL-25,26,53,54}
• Gene: Trp53 Location: Chr11:69471185-69482699 bp, + strand Genetic Position: Chr11, 42.83 cM, cytoband B2-C
• Alliance: Trp53^tm4Att page

Mutation origin

• Germline Transmission: Earliest citation of germline transmission: J:173395
• Parent Cell Line: J1 (ES Cell)
• Strain of Origin: 129S4/SvJae

Mutation description

• Allele Type: Targeted (Conditional ready, Null/knockout)
• Mutations: Insertion, Nucleotide substitutions
• Mutation details: A loxP site flanked puromycin resistance gene/transcription stop cassette was inserted into intron 1 and mutations were created in exon 2 altering codon 25 from leucine to glutamine (p.L25Q) and codon 26 from tryptophan to serine (p.W26S). Further changes in exon 4 altered codon 53 from phenylalanine to glutamine (p.F53Q) and codon 54 from phenylalanine to serine (p.F54S). Both transactivation domains are incapacitated once the stop sequences are removed by cre expression. (J:173395)

Disease models

Expression

In Structures Affected by this Mutation:

17 anatomical structure(s)

Tumor Data

List all tumor models in MMHCdb carrying Trp53^tm4Att

Find Mice (IMSR)

• Mouse strains and cell lines available from the International Mouse Strain Resource (IMSR)
• Carrying this Mutation: Mouse Strains: 1 strain available Cell Lines: 0 lines available
• Carrying any Trp53 Mutation: 240 strains or lines available

References

• Original: J:173395 Brady CA, et al., Distinct p53 transcriptional programs dictate acute DNA-damage responses and tumor suppression. Cell. 2011 May 13;145(4):571-83
• All: 7 reference(s)