Rr27^tm6.1Msb
Targeted Allele Detail

Summary

• Symbol: Rr27^tm6.1Msb
• Name: regulatory region 27; targeted mutation 6.1, Marisa S Bartolomei
• MGI ID: MGI:5140189
• Synonyms: H19^ICR-8nrCG, H19^tm9.1Msb
• Gene: Rr27 Location: Chr7:142133885-142136283 bp Genetic Position: Chr7, Syntenic
• Alliance: Rr27^tm6.1Msb page

Mutation origin

• Germline Transmission: Earliest citation of germline transmission: J:173610
• Parent Cell Line: E14.1 (ES Cell)
• Strain of Origin: 129P2/OlaHsd

Mutation description

• Allele Type: Targeted (Modified regulatory region)
• Mutations: Insertion, Intragenic deletion
• Mutation details: Site-directed mutagenesis destroyed the eight CpG islands outside of the CTCF sites. A floxed neomycin resistance gen cassette inserted downstream of the modified sequence was removed by cre-mediated recombination removed. The CpG content of the imprinting control region (ICR) is reduced 16% without changing the size of the ICR or disrupting the CTCF sites. (J:173610)

Find Mice (IMSR)

• Mouse strains and cell lines available from the International Mouse Strain Resource (IMSR)
• Carrying this Mutation: Mouse Strains: 1 strain available Cell Lines: 0 lines available
• Carrying any Rr27 Mutation: 7 strains or lines available

References

• Original: J:173610 Ideraabdullah FY, et al., Novel cis-regulatory function in ICR-mediated imprinted repression of H19. Dev Biol. 2011 Jul 15;355(2):349-57
• All: 1 reference(s)