Ttc8^tm1Reed
Targeted Allele Detail

Summary

• Symbol: Ttc8^tm1Reed
• Name: tetratricopeptide repeat domain 8; targeted mutation 1, Randall R Reed
• MGI ID: MGI:5140723
• Synonyms: Bbs8^-
• Gene: Ttc8 Location: Chr12:98886833-98949497 bp, + strand Genetic Position: Chr12, 49.88 cM
• Alliance: Ttc8^tm1Reed page

Structural and protein localization defects in Ttc8^tm1Reed/Ttc8^tm1Reed olfactory epithelium

Show the 4 phenotype image(s) involving this allele.

Mutation origin

• Germline Transmission: Earliest citation of germline transmission: J:173465
• Parent Cell Line: Not Specified (ES Cell)
• Strain of Origin: 129

Mutation description

Allele Type: Targeted (Null/knockout, Reporter) Mutations: Insertion, Intragenic deletion Mutation details: A 15.8 kb fragment including the start codon and first 2 exons was replaced with a tau/YFP fusion construct and a floxed neo cassette via homologous recombination. In situ and immunofluorescence analysis confirmed the absence of detectable RNA and protein expression in homozygous mice. Robust reporter expression is seen in sites of endogenous gene expression (microtubules, axon bundles and the nerve and glomerular layers of the olfactory bulb). (J:173465)

Generation of the Ttc8^tm1Reed allele

Disease models

Expression

In Mice Carrying this Mutation:	3 RNA-Seq or microarray experiment(s)
In Structures Affected by this Mutation:	4 anatomical structure(s)

Find Mice (IMSR)

• Mouse strains and cell lines available from the International Mouse Strain Resource (IMSR)
• Carrying this Mutation: Mouse Strains: 0 strains available Cell Lines: 0 lines available
• Carrying any Ttc8 Mutation: 34 strains or lines available

References

• Original: J:173465 Tadenev AL, et al., Loss of Bardet-Biedl syndrome protein-8 (BBS8) perturbs olfactory function, protein localization, and axon targeting. Proc Natl Acad Sci U S A. 2011 Jun 21;108(25):10320-5
• All: 6 reference(s)