Unc93b1^tm1.1Kmiy
Targeted Allele Detail

Summary

• Symbol: Unc93b1^tm1.1Kmiy
• Name: unc-93 homolog B1, TLR signaling regulator; targeted mutation 1.1, Kensuke Miyake
• MGI ID: MGI:5140947
• Synonyms: Unc93b1^D34A
• Gene: Unc93b1 Location: Chr19:3985222-3999340 bp, + strand Genetic Position: Chr19, 3.65 cM
• Alliance: Unc93b1^tm1.1Kmiy page

Mutation origin

• Germline Transmission: Earliest citation of germline transmission: J:174308
• Parent Cell Line: EB3 (ES Cell)
• Strain of Origin: 129P2/OlaHsd

Mutation description

• Allele Type: Targeted (Not Applicable)
• Mutations: Insertion, Single point mutation
• Mutation details: Exon 2 was replaced with one in which an A to C mutation results in the amino acid substitution of alanine for aspartic acid at position 34 (D34A). A floxed neo cassette inserted downstream of exon 3 was removed by cre-mediated recombination. (J:174308)

Expression

In Structures Affected by this Mutation:

4 anatomical structure(s)

Find Mice (IMSR)

• Mouse strains and cell lines available from the International Mouse Strain Resource (IMSR)
• Carrying this Mutation: Mouse Strains: 6 strains available Cell Lines: 0 lines available
• Carrying any Unc93b1 Mutation: 40 strains or lines available

Notes

ES cell line = EB3, derived from E14TG2a. EB3 cells carry one allele of Pou5f1 that has been inactivated via targeted integration of E14TG2a ES cells with an IRESBSDpA selection cassette. J:174308 does not mention whether the null allele of Pou5f1 is also present in the line of mice created.

References

• Original: J:174308 Fukui R, et al., Unc93B1 Restricts Systemic Lethal Inflammation by Orchestrating Toll-like Receptor 7 and 9 Trafficking. Immunity. 2011 Jul 22;35(1):69-81
• All: 4 reference(s)