Klf5^tm1.1Jwel
Targeted Allele Detail

Summary

• Symbol: Klf5^tm1.1Jwel
• Name: Kruppel-like transcription factor 5; targeted mutation 1.1, James Wells
• MGI ID: MGI:5141245
• Synonyms: KD, Klf5^del
• Gene: Klf5 Location: Chr14:99536127-99550848 bp, + strand Genetic Position: Chr14, 49.43 cM, cytoband E2.1
• Alliance: Klf5^tm1.1Jwel page

Mutation origin

• Germline Transmission: Earliest citation of germline transmission: J:168150
• Parent Cell Line: Not Specified (ES Cell)
• Strain of Origin: Not Specified

Mutation description

• Allele Type: Targeted (Null/knockout)
• Mutations: Insertion, Intragenic deletion
• Mutation details: A loxP flanked Hprt selection cassette upstream of exon1 and an additional loxP site downstream of exon 2 are inserted by homologous recombination. Transient Cre expression deleted exons 1 and 2. Although the cre-mediated deletion is predicted to result in the production of an in-frame transcript, the gene product from this allele is predicted to be non functional. Loss of protein was confirmed by immunostaining in early embryos. (J:168150)

Expression

In Structures Affected by this Mutation:

1 anatomical structure(s)

Find Mice (IMSR)

• Mouse strains and cell lines available from the International Mouse Strain Resource (IMSR)
• Carrying this Mutation: Mouse Strains: 0 strains available Cell Lines: 0 lines available
• Carrying any Klf5 Mutation: 37 strains or lines available

References

• Original: J:168150 Lin SC, et al., Klf5 regulates lineage formation in the pre-implantation mouse embryo. Development. 2010 Dec;137(23):3953-63
• All: 1 reference(s)