Slc32a1^tm2(cre)Lowl
Targeted Allele Detail

Summary

• Symbol: Slc32a1^tm2(cre)Lowl
• Name: solute carrier family 32 (GABA vesicular transporter), member 1; targeted mutation 2, Bradford B Lowell
• MGI ID: MGI:5141270
• Synonyms: VGAT-Cre, vGAT^iresCre, Vgat-ires-Cre
• Gene: Slc32a1 Location: Chr2:158452687-158457668 bp, + strand Genetic Position: Chr2, 78.72 cM, cytoband H1-3
• Alliance: Slc32a1^tm2(cre)Lowl page

Mutation origin

• Germline Transmission: Earliest citation of germline transmission: J:174691
• Parent Cell Line: W4 (ES Cell)
• Strain of Origin: 129S6/SvEvTac

Mutation description

• Allele Type: Targeted (Recombinase)
• Mutation: Insertion
• Slc32a1^tm2(cre)Lowl expression driven by 1 gene
  Knock-in expression driven by:
  

  Organism	Driver Gene	Note
  mouse	Slc32a1 (MGI:1194488)

• Mutation details: A targeting vector, constructed from a BAC clone containing the Slc32a1 (Vgat) gene, was utilized to insert an internal ribosomal entry site followed by the cre recombinase sequence into the Slc32a1 locus just downstream of the endogenous stop codon. Cre is expressed from the Slc32a1 gene promoter. Immunohistochemistry on brain and Western blot analysis shows decreased expression of SLC32A1 protein in homozygotes. (J:174691, J:308516)

Recombinase activity

Activity:	Tissue activity of this recombinase allele
Driver:	Slc32a1 (mouse) Summary of all recombinase alleles driven by Slc32a1. Comparative matrix view of recombinase activities Alliance mouse Slc32a1 gene page

Disease models

Expression

In Mice Carrying this Mutation:

208072 assay results 4 RNA-Seq or microarray experiment(s)

Find Mice (IMSR)

• Mouse strains and cell lines available from the International Mouse Strain Resource (IMSR)
• Carrying this Mutation: Mouse Strains: 2 strains available Cell Lines: 0 lines available
• Carrying any Slc32a1 Mutation: 55 strains or lines available

References

• Original: J:174691 Vong L, et al., Leptin Action on GABAergic Neurons Prevents Obesity and Reduces Inhibitory Tone to POMC Neurons. Neuron. 2011 Jul 14;71(1):142-54
• All: 436 reference(s)