Trp53^tm2.1Snj
Targeted Allele Detail

Summary

• Symbol: Trp53^tm2.1Snj
• Name: transformation related protein 53; targeted mutation 2.1, Stephen N Jones
• MGI ID: MGI:5142476
• Synonyms: p53^NLS1
• Gene: Trp53 Location: Chr11:69471185-69482699 bp, + strand Genetic Position: Chr11, 42.83 cM, cytoband B2-C
• Alliance: Trp53^tm2.1Snj page

Mutation origin

• Germline Transmission: Earliest citation of germline transmission: J:175018
• Parent Cell Line: PC3 (ES Cell)
• Strain of Origin: 129S4/SvJae-Tg(Prm-cre)70Og

Mutation description

• Allele Type: Targeted (Not Applicable)
• Mutations: Insertion, Nucleotide substitutions
• Mutation details: Exon 9 was replaced with a modified exon 9 in which nucleotide substitutions (AAGAAAAA>GCGGCCGC) result in the amino acid substitution of lysine with alanine at positions 316, 317, and 318. These substitutions disrupt nuclear localization signal 1 (NLS1). A loxP site flanked neomycin resistance gene cassette inserted downstream of the modified exon 9 was removed by Cre-mediated recombination. (J:175018)

Expression

In Structures Affected by this Mutation:

3 anatomical structure(s)

Find Mice (IMSR)

• Mouse strains and cell lines available from the International Mouse Strain Resource (IMSR)
• Carrying this Mutation: Mouse Strains: 0 strains available Cell Lines: 0 lines available
• Carrying any Trp53 Mutation: 239 strains or lines available

References

• Original: J:175018 Regeling A, et al., Mice defective in p53 nuclear localization signal 1 exhibit exencephaly. Transgenic Res. 2011 Aug;20(4):899-912
• All: 1 reference(s)