Sema5a^tm1.2Alk
Targeted Allele Detail

Summary

• Symbol: Sema5a^tm1.2Alk
• Name: sema domain, seven thrombospondin repeats (type 1 and type 1-like), transmembrane domain (TM) and short cytoplasmic domain, (semaphorin) 5A; targeted mutation 1.2, Alex L Kolodkin
• MGI ID: MGI:5284881
• Synonyms: Sema5A^-
• Gene: Sema5a Location: Chr15:32244959-32696487 bp, + strand Genetic Position: Chr15, 13.02 cM
• Alliance: Sema5a^tm1.2Alk page

Mutation origin

• Germline Transmission: Earliest citation of germline transmission: J:174679
• Parent Cell Line: Not Specified (ES Cell)
• Strain of Origin: 129

Mutation description

• Allele Type: Targeted (Null/knockout)
• Mutations: Insertion, Intragenic deletion
• Mutation details: A loxP site was inserted upstream of exon 6 and an FRT flanked inverted neo cassette and second loxP site were inserted downstream of exon 6 via homologous recombination. Recombinase mediated recombination removed the neo cassette and exon 6. Western blot analysis confirmed the absence of protein expression in homozygous mice. (J:174679)

Find Mice (IMSR)

• Mouse strains and cell lines available from the International Mouse Strain Resource (IMSR)
• Carrying this Mutation: Mouse Strains: 1 strain available Cell Lines: 0 lines available
• Carrying any Sema5a Mutation: 67 strains or lines available

References

• Original: J:174679 Matsuoka RL, et al., Class 5 transmembrane semaphorins control selective Mammalian retinal lamination and function. Neuron. 2011 Aug 11;71(3):460-73
• All: 3 reference(s)