Tdg^tm2.1Abc
Targeted Allele Detail

Summary

• Symbol: Tdg^tm2.1Abc
• Name: thymine DNA glycosylase; targeted mutation 2.1, Alfonso Bellacosa
• MGI ID: MGI:5285106
• Synonyms: Tdg^N151A
• Gene: Tdg Location: Chr10:82465662-82486633 bp, + strand Genetic Position: Chr10, 39.72 cM
• Alliance: Tdg^tm2.1Abc page

Mutation origin

• Germline Transmission: Earliest citation of germline transmission: J:174772
• Parent Cell Line: R1 (ES Cell)
• Strain of Origin: (129X1/SvJ x 129S1/Sv)F1-Kitl⁺

Mutation description

• Allele Type: Targeted (Not Specified)
• Mutations: Insertion, Nucleotide substitutions
• Mutation details: An FRT-flanked neo cassette with a 5' loxP site was inserted upstream of exon 3. Exon 4 was replaced with a modified exon 4 that contains nucleotide substitutions (AAC to GCC) that result in the amino acid substitution of alanine for asparagine at position 151 (N151A). An additional loxP site was inserted upstream of exon 7. Flp-mediated recombination removed the neo cassette. (J:174772)

Expression

In Structures Affected by this Mutation:

1 anatomical structure(s)

Find Mice (IMSR)

• Mouse strains and cell lines available from the International Mouse Strain Resource (IMSR)
• Carrying this Mutation: Mouse Strains: 0 strains available Cell Lines: 0 lines available
• Carrying any Tdg Mutation: 23 strains or lines available

References

• Original: J:174772 Cortellino S, et al., Thymine DNA glycosylase is essential for active DNA demethylation by linked deamination-base excision repair. Cell. 2011 Jul 8;146(1):67-79
• All: 2 reference(s)