Nrl^{tm1.1(Thrb)Df}
Targeted Allele Detail

Summary

• Symbol: Nrl^{tm1.1(Thrb)Df}
• Name: neural retina leucine zipper gene; targeted mutation 1.1, Douglas Forrest
• MGI ID: MGI:5285194
• Synonyms: Nrl^b2
• Gene: Nrl Location: Chr14:55756973-55762438 bp, - strand Genetic Position: Chr14, 28.19 cM
• Alliance: Nrl^{tm1.1(Thrb)Df} page

Mutation origin

• Germline Transmission: Earliest citation of germline transmission: J:174592
• Parent Cell Line: Not Specified (ES Cell)
• Strain of Origin: C57BL/6

Mutation description

• Allele Type: Targeted
• Mutations: Insertion, Intragenic deletion
• Mutation details: The entire coding region was replaced with an in-frame cDNA for mouse Thrb2 and a floxed neo cassette via homologous recombination. Cre mediated recombination removed the neo cassette. Western blot analysis indicated protracted expression of Thrb2 and absence of expression of Nrl in mice at E17.5. In situ hybridization showed ectopic expression of Thrb mRNA over the outer neuroblastic layer of the retina at P7 in homozygous and heterozygous mice. (J:174592)

Expression

In Structures Affected by this Mutation:

2 anatomical structure(s)

Find Mice (IMSR)

• Mouse strains and cell lines available from the International Mouse Strain Resource (IMSR)
• Carrying this Mutation: Mouse Strains: 0 strains available Cell Lines: 0 lines available
• Carrying any Nrl Mutation: 18 strains or lines available

References

• Original: J:174592 Ng L, et al., Two transcription factors can direct three photoreceptor outcomes from rod precursor cells in mouse retinal development. J Neurosci. 2011 Aug 3;31(31):11118-25
• All: 1 reference(s)