Tg(Dppa3-cre/Esr1*)3Sait
Transgene Detail

Summary

• Symbol: Tg(Dppa3-cre/Esr1*)3Sait
• Name: transgene insertion 3, Mitinori Saitou
• MGI ID: MGI:5285344
• Synonyms: Dppa3-MCM, Stella-MerCreMer
• Transgene: Tg(Dppa3-cre/Esr1*)3Sait Location: unknown
• Alliance: Tg(Dppa3-cre/Esr1*)3Sait page

Transgene origin

• Strain of Origin: (C57BL/6 x DBA)F2

Transgene description

• Transgene Type: Transgenic (Inducible, Recombinase)
• Inducer: tamoxifen
• Mutation: Insertion
• Tg(Dppa3-cre/Esr1*)3Sait expression driven by 1 gene
  Transgene expression driven by:
  

  Organism	Driver Gene	Note
  Not Specified	Dppa3

• Mutation details: A transgenic construct containing the MCM sequence (cre recombinase fused to two mutated mouse estrogen receptors) with a 3xFLAG (3F) epitope tag at the N-terminus was inserted after the initial ATG in exon 2 of Dppa3 by BAC recombineering. The construct also included the SV40 polyadenylation signal. 2 further rounds of Red/ET recombination resulted in the deletion of genes upstream (Gdf3, Apobec1, Aicda, and Mfap5) and genes downstream (Gm8460, Gm10224, Nanog, and Slc2a3) of Dppa3 in the BAC clone. The final construct contained 16 kp upstream sequence and 18 kb downstream sequence of the Dppa locus and was injected into pronuclei of B6DBA F2 zygotes. Two lines with copy numbers of 4 were analyzed and produced similar results. Line 4 was established. Lines with low copy numbers did not demonstrate efficient recombination. (J:175289)

Recombinase activity

Activity:	Tissue activity of this recombinase allele
Driver:	Dppa3 (species not specified) Summary of all recombinase alleles driven by Dppa3.

Find Mice (IMSR)

• Mouse strains and cell lines available from the International Mouse Strain Resource (IMSR)
• Carrying this Mutation: Mouse Strains: 0 strains available Cell Lines: 0 lines available

References

• Original: J:175289 Hirota T, et al., Drug-inducible gene recombination by the Dppa3-MER Cre MER transgene in the developmental cycle of the germ cell lineage in mice. Biol Reprod. 2011 Aug;85(2):367-77
• All: 4 reference(s)