Nes^tm1Nagy
Targeted Allele Detail

Summary

• Symbol: Nes^tm1Nagy
• Name: nestin; targeted mutation 1, Andras Nagy
• MGI ID: MGI:5285585
• Gene: Nes Location: Chr3:87878400-87887758 bp, + strand Genetic Position: Chr3, 38.78 cM
• Alliance: Nes^tm1Nagy page

Mutation origin

• Germline Transmission: Earliest citation of germline transmission: J:175241
• Parent Cell Line: G4 (ES Cell)
• Strain of Origin: (129S6/SvEvTac x C57BL/6NCrl)F1

Mutation description

• Allele Type: Targeted (Null/knockout)
• Mutations: Insertion, Intragenic deletion
• Mutation details: Part of the 5' region and almost all of exon 1 were replaced with a pgk-neo cassette via homologous recombination. Western blot analysis confirmed the absence of protein expression in homozygous embryos. (J:175241)

Find Mice (IMSR)

• Mouse strains and cell lines available from the International Mouse Strain Resource (IMSR)
• Carrying this Mutation: Mouse Strains: 0 strains available Cell Lines: 0 lines available
• Carrying any Nes Mutation: 82 strains or lines available

References

• Original: J:175241 Mohseni P, et al., Nestin Is Not Essential for Development of the CNS But Required for Dispersion of Acetylcholine Receptor Clusters at the Area of Neuromuscular Junctions. J Neurosci. 2011 Aug 10;31(32):11547-11552
• All: 4 reference(s)