Myh9^tm8.1Rsad
Targeted Allele Detail

Summary

• Symbol: Myh9^tm8.1Rsad
• Name: myosin, heavy polypeptide 9, non-muscle; targeted mutation 8, Robert S Adelstein
• MGI ID: MGI:5285645
• Synonyms: A^gfp, GFP-NMHCII-A, GFP-NMII-A
• Gene: Myh9 Location: Chr15:77644788-77726315 bp, - strand Genetic Position: Chr15, 36.81 cM
• Alliance: Myh9^tm8.1Rsad page

Mutation origin

• Germline Transmission: Earliest citation of germline transmission: J:175367
• Parent Cell Line: v6.5 (ES Cell)
• Strain of Origin: (C57BL/6 x 129S4/SvJae)F1

Mutation description

• Allele Type: Targeted (Null/knockout, Reporter)
• Mutation: Insertion
• Mutation details: A cDNA encoding enhanced green fluorescent protein was inserted 5' of the initiating ATG codon in exon 2 and a PGK-neo^r cassette into intron 2 of the myosin, heavy polypeptide 9, non-muscle (Myh9) gene. The selection cassette has been deleted by Cre recombinase-mediated excision. Immunoblot analysis demonstrates that levels of wild-type and EGFP-tagged MYH9 protein are similar in lungs of heterozygous mutant mice. (J:175367)
• Inheritance: Semidominant

Find Mice (IMSR)

• Mouse strains and cell lines available from the International Mouse Strain Resource (IMSR)
• Carrying this Mutation: Mouse Strains: 1 strain available Cell Lines: 0 lines available
• Carrying any Myh9 Mutation: 219 strains or lines available

References

• Original: J:175367 Zhang Y, et al., Mouse models of MYH9-related disease: mutations in nonmuscle myosin II-A. Blood. 2012;119(1):238-5
• All: 11 reference(s)