Gt(ROSA)26Sor^{tm1.1(rtTA,EGFP)Nagy}
Targeted Allele Detail

Summary

• Symbol: Gt(ROSA)26Sor^{tm1.1(rtTA,EGFP)Nagy}
• Name: gene trap ROSA 26, Philippe Soriano; targeted mutation 1.1, Andras Nagy
• MGI ID: MGI:5286599
• Synonyms: ROSA26rtTA
• Gene: Gt(ROSA)26Sor Location: Chr6:113044389-113054205 bp, - strand Genetic Position: Chr6, 52.73 cM
• Alliance: Gt(ROSA)26Sor^{tm1.1(rtTA,EGFP)Nagy} page

Mutation origin

• Germline Transmission: Earliest citation of germline transmission: J:174960
• Parent Cell Line: R1 (ES Cell)
• Strain of Origin: (129X1/SvJ x 129S1/Sv)F1-Kitl⁺

Mutation description

• Allele Type: Targeted (Reporter, Transactivator)
• Mutation: Insertion
• Mutation details: A targeting vector containing a floxed Pgk-neo-pA cassette and a rtTA-IRES-EGFP-pA cassette was inserted into intron 1 of the locus. Cre-mediated recombination removed the floxed neo cassette allowing the ROSA26 promoter to drive expression of rtTA and EGFP. Presence of doxycycline results in the formation of an active transcriptional activator and the activation of the responder transgene. (J:174960)

Find Mice (IMSR)

• Mouse strains and cell lines available from the International Mouse Strain Resource (IMSR)
• Carrying this Mutation: Mouse Strains: 6 strains available Cell Lines: 0 lines available
• Carrying any Gt(ROSA)26Sor Mutation: 993 strains or lines available

References

• Original: J:174960 Le TT, et al., Temporal requirement for high SMN expression in SMA mice. Hum Mol Genet. 2011 Sep 15;20(18):3578-91
• All: 12 reference(s)