Syt10^{tm1.1(icre)Geno}
Targeted Allele Detail

Summary

• Symbol: Syt10^{tm1.1(icre)Geno}
• Name: synaptotagmin X; targeted mutation 1.1, Genoway
• MGI ID: MGI:5286607
• Synonyms: Syt10^Cre
• Gene: Syt10 Location: Chr15:89666596-89726063 bp, - strand Genetic Position: Chr15, 44.96 cM, cytoband F1
• Alliance: Syt10^{tm1.1(icre)Geno} page

Mutation origin

• Germline Transmission: Earliest citation of germline transmission: J:175596
• Parent Cell Line: Not Specified (ES Cell)
• Strain of Origin: 129S2/SvPas

Mutation description

• Allele Type: Targeted (Recombinase)
• Mutations: Insertion, Intragenic deletion
• Syt10^{tm1.1(icre)Geno} expression driven by 1 gene
  Knock-in expression driven by:
  

  Organism	Driver Gene	Note
  mouse	Syt10 (MGI:1859546)

• Mutation details: A targeting construct containing cre recombinase, an IRES, and cDNA encoding EGFP replaced the Syt10 exon 1 sequence following the initiating ATG codon in ES cells. An Frt-flanked neomycin selection cassette after the EGFP sequence was also included. This was subsequently deleted from F1 animals by crossing to Gt(ROSA)26Sor^tm1(FLP1)Dym mice on a C57BL/6 background. No transcripts of Syt10 are detected in the suprachiasmatic nuclei or other sites of endogenous expression in the brain. Breeding experiments show that cre is active in the male germline. (J:175596)

Recombinase activity

Activity:	Tissue activity of this recombinase allele
Driver:	Syt10 (mouse) Summary of all recombinase alleles driven by Syt10. Alliance mouse Syt10 gene page

Expression

In Mice Carrying this Mutation:

3 RNA-Seq or microarray experiment(s)

Find Mice (IMSR)

• Mouse strains and cell lines available from the International Mouse Strain Resource (IMSR)
• Carrying this Mutation: Mouse Strains: 0 strains available Cell Lines: 0 lines available
• Carrying any Syt10 Mutation: 35 strains or lines available

References

• Original: J:175596 Husse J, et al., Synaptotagmin10-Cre, a Driver to Disrupt Clock Genes in the SCN. J Biol Rhythms. 2011 Oct;26(5):379-89
• All: 5 reference(s)