Zfp335^{Tg(Tyr)2359A-4d2Ove}
Transgenic Allele Detail

Summary

• Symbol: Zfp335^{Tg(Tyr)2359A-4d2Ove}
• Name: zinc finger protein 335; transgene lentiviral insertion 2359A-4d2, Paul A Overbeek
• MGI ID: MGI:5286608
• Synonyms: OVE#2359A-4d2, OVE2359A-4d2
• Gene: Zfp335 Location: Chr2:164733802-164753677 bp, - strand Genetic Position: Chr2, 85.27 cM
• Alliance: Zfp335^{Tg(Tyr)2359A-4d2Ove} page

Transgene origin

• Strain of Origin: FVB/N

Transgene description

• Transgene Type: Transgenic (Reporter)
• Mutation: Insertion
• Mutation details: The Tyro-IRES-sd-loxP-FUGW lentiviral transgene (LV2187) was designed with a mouse tyrosinase minigene (Tyro) followed by an IRES::3xATG::splice-donor::loxP sequence in the FUGW self-inactivating HIV-based lentiviral vector backbone. The Tyro-IRES-sd-loxP replaced the ubiquitin-c promoter, EGFP, and WPRE sequences originally found in the FUGW lentiviral vector. The Tyro minigene is composed of the mouse Tyr enhancer region (623 bp), promoter region (657 bp), and 1566 bp cDNA sequence (including the stop codon); all in antisense orientation relative to the FUGW backbone. The Tyro minigene is followed by an internal ribosome entry site (IRES) ending with ATGs in all three reading frames (3xATG), an artificial splice donor sequence, and a loxP site in this construct. In line 2359A-4d2, the lentiviral transgene inserted into exon 8 (NCBI37/mm9; 3'-164,728,108(-)). (J:175597)

Find Mice (IMSR)

• Mouse strains and cell lines available from the International Mouse Strain Resource (IMSR)
• Carrying this Mutation: Mouse Strains: 0 strains available Cell Lines: 0 lines available
• Carrying any Zfp335 Mutation: 67 strains or lines available

References

• Original: J:175597 Overbeek PA, Direct Data Submission for Overbeek Lentiviral Transgenic Lines. MGI Direct Data Submission. 2011
• All: 1 reference(s)