In(10C1;Ccdc53)4Ove
Transposon induced Allele Detail

Summary

• Symbol: In(10C1;Ccdc53)4Ove
• Name: inversion, Chr 10, Paul A Overbeek 4
• MGI ID: MGI:5287112
• Synonyms: In(10)2522A-SB2Ove, In4Ove, OVE#2522A-SB2, OVE2522A-SB2
• Gene: In(10C1;Ccdc53)4Ove Location: unknown Genetic Position: Chr10, Syntenic

Mutation origin

• Strain of Origin: FVB/N

Mutation description

• Allele Type: Transposon induced
• Mutations: Inversion, Transposon insertion
• In(10C1;Ccdc53)4Ove involves 132 genes/genome features (Gm26546, Gm26700, Gm25266 ...) View all
• Mutation details: The SB-cHS4core-SB-Tyro-WPRE-FUGW lentiposon transgene (LV2229) was designed with a Sleeping Beauty (SB) transposon (inward-facing SB arms flanking a tandem pair of cHS4 core insulator elements), followed by a mouse tyrosinase minigene (Tyro) and a woodchuck hepatitis virus post-transcriptional regulatory element (WPRE) in the FUGW self-inactivating HIV-based lentiviral vector backbone. The SB transposon and Tyro minigene replaced the ubiquitin-c promoter and EGFP sequences originally found in the FUGW lentiviral vector. The SB transposon used in this transgene has an inverted repeat/direct repeat sequence (IR/DR; the SB transposon recognition site), two copies of the 0.25 kb chicken beta-globin core insulator element (cHS4 core), and a second IR/DR sequence. The IR/DR sequences are inward-facing (pointed towards the cHS4core). The cHS4 core insulator element functions to terminate transcription of the upstream locus into which it integrates (and also enhance expression from the downstream Tyro minigene). The Tyro minigene is composed of the mouse Tyr enhancer region (623 bp), promoter region (657 bp), and 1566 bp cDNA sequence (including the stop codon); all in sense orientation relative to the FUGW backbone. There is no polyA site between the Tyro minigene and WPRE sequence. The WPRE sequence functions to enhance the mRNA transcript stability. The transposon was mobilized by a testes-specific SB10 transposase. In line OVE2522A-SB2, the lentiviral integration results in an inversion between an agenic region in chromosome 10 C1 [NCB137/mm9; 3'-95,095,943(+)] and integration site at intron 5 of Ccdc53 [NCBI37/mm9; L3-87,696,733(-)]. (J:175597)

Expression

In Structures Affected by this Mutation:

1 anatomical structure(s)

Find Mice (IMSR)

• Mouse strains and cell lines available from the International Mouse Strain Resource (IMSR)
• Carrying this Mutation: Mouse Strains: 1 strain available Cell Lines: 0 lines available
• Carrying any In(10C1;Ccdc53)4Ove Mutation: 1 strain or line available

References

• Original: J:175597 Overbeek PA, Direct Data Submission for Overbeek Lentiviral Transgenic Lines. MGI Direct Data Submission. 2011
• All: 1 reference(s)