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PoleTg(Tyr)2300A-1b1b1Ove
Transgenic Allele Detail
Summary
Symbol: PoleTg(Tyr)2300A-1b1b1Ove
Name: polymerase (DNA directed), epsilon; transgene lentiviral insertion 23000A-2b1b1, Paul A Overbeek
MGI ID: MGI:5287491
Synonyms: OVE#23000A-1b1b, OVE23000A-1b1b
Gene: Pole  Location: Chr5:110434185-110485319 bp, + strand  Genetic Position: Chr5, 53.45 cM, cytoband E3-E5
Alliance: PoleTg(Tyr)2300A-1b1b1Ove page
Transgene
origin
Strain of Origin:  FVB/N
Transgene
description
Transgene Type:    Transgenic (Reporter)
Mutation:    Insertion
 
Mutation detailsA lentiviral transgenic approach was used to generate these mice. The Tyro-WPRE-FUGW lentiviral transgene (LV2177) was designed with a mouse tyrosinase minigene (Tyro) and woodchuck hepatitis virus post-transcriptional regulatory element (WPRE) in the FUGW self-inactivating HIV-based lentiviral vector backbone. The Tyro minigene replaced the ubiquitin-c promoter and EGFP sequences originally found in the FUGW lentiviral vector. The Tyro minigene is composed of the mouse Tyr enhancer region (623 bp), promoter region (657 bp), and 1566 bp cDNA sequence (including the stop codon); all in sense orientation relative to the FUGW backbone. There is no polyA site between the Tyro minigene and WPRE sequence. The WPRE sequence functions to enhance the mRNA transcript stability. In line OVE2300A-1b1b, the lentivirus integrates into intron 40 [NCBI37/mm9; 3'-110,758,002(+)] in the sense orientation. (J:175597)
Phenotypes
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Find Mice (IMSR)
Mouse strains and cell lines available from the International Mouse Strain Resource (IMSR)
Carrying this Mutation:  Mouse Strains: 0 strains available      Cell Lines: 0 lines available
Carrying any Pole Mutation:  99 strains or lines available
References
Original:  J:175597 Overbeek PA, Direct Data Submission for Overbeek Lentiviral Transgenic Lines. MGI Direct Data Submission. 2011;
All:  1 reference(s)

Contributing Projects:
Mouse Genome Database (MGD), Gene Expression Database (GXD), Mouse Models of Human Cancer database (MMHCdb) (formerly Mouse Tumor Biology (MTB)), Gene Ontology (GO)
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last database update
10/22/2024
MGI 6.24
The Jackson Laboratory