Pfas^{Tg(Tyr)2307COve}
Transgenic Allele Detail

Summary

• Symbol: Pfas^{Tg(Tyr)2307COve}
• Name: phosphoribosylformylglycinamidine synthase (FGAR amidotransferase); transgene lentiviral insertion 2307C, Paul A Overbeek
• MGI ID: MGI:5287493
• Synonyms: OVE#2307C, OVE2307C
• Gene: Pfas Location: Chr11:68876527-68899286 bp, - strand Genetic Position: Chr11, 42.26 cM
• Alliance: Pfas^{Tg(Tyr)2307COve} page

Transgene origin

• Strain of Origin: FVB/N

Transgene description

• Transgene Type: Transgenic (Reporter)
• Mutation: Insertion
• Mutation details: A lentiviral transgenic approach was used to generate these mice. The Tyro-WPRE-FUGW lentiviral transgene (LV2177) was designed with a mouse tyrosinase minigene (Tyro) and woodchuck hepatitis virus post-transcriptional regulatory element (WPRE) in the FUGW self-inactivating HIV-based lentiviral vector backbone. The Tyro minigene replaced the ubiquitin-c promoter and EGFP sequences originally found in the FUGW lentiviral vector. The Tyro minigene is composed of the mouse Tyr enhancer region (623 bp), promoter region (657 bp), and 1566 bp cDNA sequence (including the stop codon); all in sense orientation relative to the FUGW backbone. There is no polyA site between the Tyro minigene and WPRE sequence. The WPRE sequence functions to enhance the mRNA transcript stability. In line OVE2307C, the lentivirus integrates into intron 1 [NCBI37/mm9; 3'-68,817,979(+)] in the antisense orientation. (J:175597)

Find Mice (IMSR)

• Mouse strains and cell lines available from the International Mouse Strain Resource (IMSR)
• Carrying this Mutation: Mouse Strains: 0 strains available Cell Lines: 0 lines available
• Carrying any Pfas Mutation: 98 strains or lines available

References

• Original: J:175597 Overbeek PA, Direct Data Submission for Overbeek Lentiviral Transgenic Lines. MGI Direct Data Submission. 2011
• All: 1 reference(s)