Prkg1^{Tn(sb-rtTA)2497B.SB2Ove}
Transposon induced Allele Detail

Summary

• Symbol: Prkg1^{Tn(sb-rtTA)2497B.SB2Ove}
• Name: protein kinase, cGMP-dependent, type I; transposon insertion 2479B.SB2, Paul A Overbeek
• MGI ID: MGI:5287505
• Synonyms: OVE#2497B-SB2, OVE2497B-SB2
• Gene: Prkg1 Location: Chr19:30541889-31742433 bp, - strand Genetic Position: Chr19, 25.17 cM, cytoband C2
• Alliance: Prkg1^{Tn(sb-rtTA)2497B.SB2Ove} page

Mutation origin

• Strain of Origin: FVB/N

Mutation description

• Allele Type: Transposon induced (Inserted expressed sequence)
• Mutation: Transposon insertion
• Mutation details: A lentiviral transgenic approach was used to generate these mice. The SB-sa-IRES-rtTA-pA-SB-Tyro-WPRE-FUGW lentiposon transgene (LV2223) was designed with a Sleeping Beauty (SB) transposon (containing a slice-acceptor::IRES::rtTA::polyA gene trap), a mouse tyrosinase minigene (Tyro), and a woodchuck hepatitis virus post-transcriptional regulatory element (WPRE) in the FUGW self-inactivating HIV-based lentiviral vector backbone. The SB transposon and Tyro minigene replaced the ubiquitin-c promoter and EGFP sequences originally found in the FUGW lentiviral vector. The 1200 bp SB transposon used in this transgene has an inverted repeat/direct repeat sequence (IR/DR; the SB transposon recognition site), an adenovirus splice acceptor, a stop sequence (3xSTOP), an internal ribosome entry site (IRES; from human X-chromosome-linked inhibitor of apoptosis (XIAP)), a sequence encoding a-n optimized form of reverse tetracycline controlled transactivator (rtTA2S; with stop codon), a human growth hormone polyA sequence, and a second IR/DR sequence. The IR/DR sequences are outward-facing (pointed away from the sa-IRES-rtTA-pA). The Tyro minigene is composed of the mouse Tyr enhancer region (623 bp), promoter region (657 bp), and 1566 bp cDNA sequence (including the stop codon); all in sense orientation relative to the FUGW backbone. There is no polyA site between the Tyro minigene and WPRE sequence. The WPRE sequence functions to enhance the mRNA transcript stability. The transposon was mobilized by a testes-specific SB10. In line OVE2497B-SB2, the lentiviral integrated into intron 10 [NCBI37/mm9; ?-30,692,871(-)] in the sense orientation. (J:175597)

Find Mice (IMSR)

• Mouse strains and cell lines available from the International Mouse Strain Resource (IMSR)
• Carrying this Mutation: Mouse Strains: 1 strain available Cell Lines: 0 lines available
• Carrying any Prkg1 Mutation: 60 strains or lines available

References

• Original: J:175597 Overbeek PA, Direct Data Submission for Overbeek Lentiviral Transgenic Lines. MGI Direct Data Submission. 2011
• All: 3 reference(s)