Tg(Rnu6-RNAi:Bccip)4Zshn
Transgene Detail

Summary

• Symbol: Tg(Rnu6-RNAi:Bccip)4Zshn
• Name: transgene insertion 4, Zhiyuan Shen
• MGI ID: MGI:5288517
• Synonyms: LoxPshBCCIP-4
• Transgene: Tg(Rnu6-RNAi:Bccip)4Zshn Location: unknown
• Alliance: Tg(Rnu6-RNAi:Bccip)4Zshn page

Transgene origin

• Strain of Origin: FVB/N

Transgene description

• Transgene Type: Transgenic (Knockdown)
• Mutation: Insertion
• Tg(Rnu6-RNAi:Bccip)4Zshn involves 1 genes/genome features (Bccip) View all
• Mutation details: A knockdown vector was constructed with a conditional Rnu6 (U6) promoter (disrupted by insertion of a loxP-flanked neo cassette) driving short hairpin RNA specific for mouse Bccip. The construct was injected into FVB/N fertilized oocytes. Two founder lines (4 and 13) were generated. Line 4 was analyzed to a greater extent. Cre recombination will excise the floxed neo cassette, reconstitutng activity of the Rnu6 promoter and allowing transcription of the anti-Bccip shRNA resulting in RNA interference of the Bccip gene. (J:176115)

Expression

In Structures Affected by this Mutation:

4 anatomical structure(s)

Find Mice (IMSR)

• Mouse strains and cell lines available from the International Mouse Strain Resource (IMSR)
• Carrying this Mutation: Mouse Strains: 0 strains available Cell Lines: 0 lines available

References

• Original: J:176115 Lu H, et al., Essential Roles of BCCIP in Mouse Embryonic Development and Structural Stability of Chromosomes. PLoS Genet. 2011 Sep;7(9):e1002291
• All: 5 reference(s)