Nrp1^tm1.1Cruh
Targeted Allele Detail

Summary

• Symbol: Nrp1^tm1.1Cruh
• Name: neuropilin 1; targeted mutation 1.1, Christiana Ruhrberg
• MGI ID: MGI:5288597
• Synonyms: Nrp1^cyto, Nrp1^cytodelta
• Gene: Nrp1 Location: Chr8:129085085-129229844 bp, + strand Genetic Position: Chr8, 75.78 cM, cytoband E
• Alliance: Nrp1^tm1.1Cruh page

Mutation origin

• Germline Transmission: Earliest citation of germline transmission: J:176047
• Parent Cell Line: Not Specified (ES Cell)
• Strain of Origin: Not Specified

Mutation description

• Allele Type: Targeted
• Mutations: Insertion, Intragenic deletion
• Mutation details: A 4 base pair insertion (AATT) was inserted into the last exon after the transmembrane domain, shifting the reading frame and preventing translation of the cytoplasmic domain. A floxed neo cassette was inserted after the last exon and then removed by cre-mediated recombination. Immunoblot analysis confirmed the deletion of the cytoplasmic domain and stable expression of the mutant protein in primary cardiac endothelial cells from homozygous mice. (J:176047)

Disease models

Expression

In Mice Carrying this Mutation:	14 assay results
In Structures Affected by this Mutation:	1 anatomical structure(s)

Find Mice (IMSR)

• Mouse strains and cell lines available from the International Mouse Strain Resource (IMSR)
• Carrying this Mutation: Mouse Strains: 2 strains available Cell Lines: 0 lines available
• Carrying any Nrp1 Mutation: 84 strains or lines available

References

• Original: J:176047 Fantin A, et al., The cytoplasmic domain of neuropilin 1 is dispensable for angiogenesis, but promotes the spatial separation of retinal arteries and veins. Development. 2011 Oct;138(19):4185-91
• All: 6 reference(s)