Gt(ROSA)26Sor^{tm4(H1/tetO-RNAi:Mir145)Jcbr}
Targeted Allele Detail

Summary

• Symbol: Gt(ROSA)26Sor^{tm4(H1/tetO-RNAi:Mir145)Jcbr}
• Name: gene trap ROSA 26, Philippe Soriano; targeted mutation 4, Jens C Bruning
• MGI ID: MGI:5290084
• Synonyms: miR-145^DOX
• Gene: Gt(ROSA)26Sor Location: Chr6:113044389-113054205 bp, - strand Genetic Position: Chr6, 52.73 cM
• Alliance: Gt(ROSA)26Sor^{tm4(H1/tetO-RNAi:Mir145)Jcbr} page

Mutation origin

• Germline Transmission: Earliest citation of germline transmission: J:176266
• Parent Cell Line: ART4.12 (ES Cell)
• Strain of Origin: (C57BL/6 x 129S6/SvEvTac)F1

Mutation description

• Allele Type: Targeted (Inducible, Knockdown, RMCE-ready)
• Inducer: doxycycline/tetracycline
• Mutation: Insertion
• Gt(ROSA)26Sor^{tm4(H1/tetO-RNAi:Mir145)Jcbr} involves 1 genes/genome features (Mir145a) View all
• Mutation details: A construct containing a short hairpin RNA targeting Mir145 driven by the H1/tetO operator fusion promoter followed by a CAG promoter driving expression of the tetR regulatory cassette was inserted into the locus via flp-mediated recombination using RMCE with Gt(ROSA)26Sor^{tm1(CAG-flpe)Arte}. Doxycycline-treatment is required to activate expression of the shRNA. (J:176266)

Find Mice (IMSR)

• Mouse strains and cell lines available from the International Mouse Strain Resource (IMSR)
• Carrying this Mutation: Mouse Strains: 0 strains available Cell Lines: 0 lines available
• Carrying any Gt(ROSA)26Sor Mutation: 993 strains or lines available

References

• Original: J:176266 Jordan SD, et al., Obesity-induced overexpression of miRNA-143 inhibits insulin-stimulated AKT activation and impairs glucose metabolism. Nat Cell Biol. 2011 Apr;13(4):434-46
• All: 1 reference(s)