Prkab1^tm1Grst
Targeted Allele Detail

Summary

• Symbol: Prkab1^tm1Grst
• Name: protein kinase, AMP-activated, beta 1 non-catalytic subunit; targeted mutation 1, Gregory R Steinberg
• MGI ID: MGI:5293373
• Synonyms: Prkab1^fl
• Gene: Prkab1 Location: Chr5:116151654-116162449 bp, - strand Genetic Position: Chr5, 56.1 cM, cytoband F
• Alliance: Prkab1^tm1Grst page

Mutation origin

• Germline Transmission: Earliest citation of germline transmission: J:176578
• Parent Cell Line: Not Specified (ES Cell)
• Strain of Origin: Not Specified

Mutation description

• Allele Type: Targeted (Conditional ready, No functional change)
• Mutation: Insertion
• Mutation details: A loxP was inserted upstream of exon 2, and a loxP site flanked neomycin resistance gene cassette was inserted into of intron 4. (J:176578)

Phenotypes

Key:

hm	homozygous	ht	heterozygous	tg	involves transgenes	√	phenotype observed
cn	conditional genotype	cx	complex: > 1 genome feature	ot	other: hemizygous, indeterminate,...	N	normal phenotype

Genotype/
Background:

	Allelic Composition	Genetic Background	Cell Line(s)
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cn1	Prkab1^tm1Grst/Prkab1^tm1Grst Prkab2^tm1Grst/Prkab2^tm1Grst Tg(Ckmm-cre)5Khn/0	involves: C57BL/6 * FVB

Phenotypes:

Affected Systems	cn1
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behavior/neurological	√
decreased locomotor activity	√
impaired exercise endurance	√
cellular	√
decreased muscle cell glucose uptake	√
homeostasis/metabolism	√
impaired exercise endurance	√
increased circulating glucose level	√
abnormal enzyme/coenzyme activity	√
muscle	√
decreased muscle cell glucose uptake	√
abnormal skeletal muscle morphology	√
increased muscle fatigability	√

View phenotypes and curated references for all genotypes (concatenated display).

Find Mice (IMSR)

• Mouse strains and cell lines available from the International Mouse Strain Resource (IMSR)
• Carrying this Mutation: Mouse Strains: 0 strains available Cell Lines: 0 lines available
• Carrying any Prkab1 Mutation: 24 strains or lines available

References

• Original: J:176578 O'Neill HM, et al., AMP-activated protein kinase (AMPK) {beta}1{beta}2 muscle null mice reveal an essential role for AMPK in maintaining mitochondrial content and glucose uptake during exercise. Proc Natl Acad Sci U S A. 2011 Sep 20;108(38):16092-7
• All: 8 reference(s)