Tg(CAG-mRFP1,-SOX9,-EGFP)1Haak
Transgene Detail

Summary

• Symbol: Tg(CAG-mRFP1,-SOX9,-EGFP)1Haak
• Name: transgene insertion 1, Haruhiko Akiyama
• MGI ID: MGI:5293615
• Synonyms: CAG-mRFP1^floxed-Sox9-EGFP, CAG-Sox9
• Transgene: Tg(CAG-mRFP1,-SOX9,-EGFP)1Haak Location: unknown
• Alliance: Tg(CAG-mRFP1,-SOX9,-EGFP)1Haak page

Transgene origin

• Strain of Origin: C57BL/6

Transgene description

• Transgene Type: Transgenic (Inserted expressed sequence)
• Mutation: Insertion
• Tg(CAG-mRFP1,-SOX9,-EGFP)1Haak expresses 1 gene
  Transgene expresses:
  

  Organism	Expressed Gene	Note
  mouse	Sox9 (MGI:98371)

• Mutation details: The transgene consists of the CAG promoter, a cassette containing a loxP-flanked mRFP1 gene and 4 tandem polyadneylation sites, the human SOX9 cDNA with a 3x hemagglutinin epitope tag, and an IRES-EGFP-polyA sequence. The vector was injected into C57BL/6 oocytes to generated mice. 8 founders were obtained and one line that showed significant levels of (ubiquitous) mRFP1 expression was chosen for further study. (J:176952)

Expression

In Structures Affected by this Mutation:

1 anatomical structure(s)

Find Mice (IMSR)

• Mouse strains and cell lines available from the International Mouse Strain Resource (IMSR)
• Carrying this Mutation: Mouse Strains: 1 strain available Cell Lines: 0 lines available

References

• Original: J:176952 Kim Y, et al., Generation of transgenic mice for conditional overexpression of Sox9. J Bone Miner Metab. 2011 Jan;29(1):123-9
• All: 11 reference(s)