Cd8a^{tm1.1(cre)Koni}
Targeted Allele Detail

Summary

• Symbol: Cd8a^{tm1.1(cre)Koni}
• Name: CD8 subunit alpha; targeted mutation 1.1, Pandelakis A Koni
• MGI ID: MGI:5294414
• Synonyms: Cd8acre
• Gene: Cd8a Location: Chr6:71350411-71356155 bp, + strand Genetic Position: Chr6, 32.14 cM, cytoband C
• Alliance: Cd8a^{tm1.1(cre)Koni} page

Mutation origin

• Germline Transmission: Earliest citation of germline transmission: J:177182
• Parent Cell Line: LK-1/LK1 (ES Cell)
• Strain of Origin: C57BL/6NHsd

Mutation description

• Allele Type: Targeted (Recombinase)
• Mutations: Insertion, Intragenic deletion
• Cd8a^{tm1.1(cre)Koni} expression driven by 1 gene
  Knock-in expression driven by:
  

  Organism	Driver Gene	Note
  mouse	Cd8a (MGI:88346)

• Mutation details: A targeting vector was designed to insert a cre-recombinase coding sequence, a neomycin resistance cassette, and an internal ribosome entry site (IRES) into the start codon of the CD8 antigen alpha chain (Cd8a) gene. The construct was electroporated into C57BL/6NHsd-derived LK1 embryonic stem (ES) cells. Chimeras were bred to C57BL/6 animals. Offspring were crossed to Flp-expressing mice to delete the neo cassette. (J:177182)

Recombinase activity

Activity:	Tissue activity of this recombinase allele
Driver:	Cd8a (mouse) Summary of all recombinase alleles driven by Cd8a. Alliance mouse Cd8a gene page

Find Mice (IMSR)

• Mouse strains and cell lines available from the International Mouse Strain Resource (IMSR)
• Carrying this Mutation: Mouse Strains: 1 strain available Cell Lines: 0 lines available
• Carrying any Cd8a Mutation: 39 strains or lines available

References

• Original: J:177182 Koni PA, Generation of a cre knock-in allele at the Cd8a locus. MGI Direct Data Submission. 2011
• All: 2 reference(s)