Col1a1^{tm3(tetO-Mirlet7g/Mir21)Gqda}
Targeted Allele Detail

Summary

• Symbol: Col1a1^{tm3(tetO-Mirlet7g/Mir21)Gqda}
• Name: collagen, type I, alpha 1; targeted mutation 3, George Q Daley
• MGI ID: MGI:5294613
• Synonyms: iLet-7, let-7, let-7S21L
• Gene: Col1a1 Location: Chr11:94827050-94843868 bp, + strand Genetic Position: Chr11, 59.01 cM, cytoband D
• Alliance: Col1a1^{tm3(tetO-Mirlet7g/Mir21)Gqda} page

Mutation origin

• Germline Transmission: Earliest citation of germline transmission: J:177113
• Parent Cell Line: KH2 (ES Cell)
• Strain of Origin: (C57BL/6 x 129S4/SvJae)F1

Mutation description

• Allele Type: Targeted (Inducible, Inserted expressed sequence, RMCE-ready)
• Inducer: doxycycline/tetracycline
• Mutation: Insertion
• Col1a1^{tm3(tetO-Mirlet7g/Mir21)Gqda} expresses 2 genes
  Knock-in expresses:
  

  Organism	Expressed Gene	Note
  mouse	Mir21a (MGI:2676885)
  mouse	Mirlet7g (MGI:2676800)

• Mutation details: RMCE on KH2 cells inserted a cassette that contains a splice acceptor, 2 polyadenylation sequence, tetracycline operator, a fusion of the Mirlet7g stem sequence and the Mir21 loop sequence, and an SV40 polyadenylation signal into the 3'UTR. No expression of the inserted sequence is detected in the absence of doxycycline treatment. (J:177113)

Find Mice (IMSR)

• Mouse strains and cell lines available from the International Mouse Strain Resource (IMSR)
• Carrying this Mutation: Mouse Strains: 1 strain available Cell Lines: 0 lines available
• Carrying any Col1a1 Mutation: 163 strains or lines available

Notes

The allele was made in KH2 cells that carry Gt(ROSA)26Sor^{tm1(rtTA*M2)Jae} and Col1a1^{tm13(neo/hygro*)Jae} (J:159351). This allele was generated with Col1a1^{tm13(neo/hygro*)Jae}.

References

• Original: J:177113 Zhu H, et al., The Lin28/let-7 Axis Regulates Glucose Metabolism. Cell. 2011 Sep 30;147(1):81-94
• All: 21 reference(s)