Lgr5^{tm2(Hbegf/EGFP)Fjs}
Targeted Allele Detail

Summary

• Symbol: Lgr5^{tm2(Hbegf/EGFP)Fjs}
• Name: leucine rich repeat containing G protein coupled receptor 5; targeted mutation 2, Frederick J de Sauvage
• MGI ID: MGI:5294798
• Synonyms: Lgr5^DTR, Lgr5DTR-EGFP, Lgr5-DTR-GFP, Lgr5-EGFP-DTR, Lgr5^{tm2(DTR/EGFP)Fjs}
• Gene: Lgr5 Location: Chr10:115286219-115423685 bp, - strand Genetic Position: Chr10, 63.51 cM
• Alliance: Lgr5^{tm2(Hbegf/EGFP)Fjs} page

Mutation origin

• Germline Transmission: Earliest citation of germline transmission: J:177145
• Parent Cell Line: C2 (Nagy) (ES Cell)
• Strain of Origin: C57BL/6NTac

Mutation description

• Allele Type: Targeted (Inserted expressed sequence, Null/knockout, Reporter)
• Mutations: Insertion, Intragenic deletion
• Lgr5^{tm2(Hbegf/EGFP)Fjs} expresses 1 gene
  Knock-in expresses:
  

  Organism	Expressed Gene	Note
  Not Specified	Hbegf

• Mutation details: A targeting vector was generated so that a DTR-EGFP-pA-loxP-neo-loxP cassette (diphtheria toxin (DT) cDNA linked in-frame to EGFP polyA-floxed neo) was inserted immediately after the ATG codon in exon 1 of the Lgr5 locus (deleting the remainder of exon 1 and splice donor site in intron 1 - a 212 bp deletion) by homologous recombination in C2 ES cells. Correctly targeted clones were transfected with an cre plasmid to remove the neo cassette. (J:177145)

Expression

In Mice Carrying this Mutation:

2 RNA-Seq or microarray experiment(s)

Find Mice (IMSR)

• Mouse strains and cell lines available from the International Mouse Strain Resource (IMSR)
• Carrying this Mutation: Mouse Strains: 1 strain available Cell Lines: 0 lines available
• Carrying any Lgr5 Mutation: 58 strains or lines available

References

• Original: J:177145 Tian H, et al., A reserve stem cell population in small intestine renders Lgr5-positive cells dispensable. Nature. 2011 Oct 13;478(7368):255-9
• All: 53 reference(s)