About   Help   FAQ
Lgr5tm2(Hbegf/EGFP)Fjs
Targeted Allele Detail
Summary
Symbol: Lgr5tm2(Hbegf/EGFP)Fjs
Name: leucine rich repeat containing G protein coupled receptor 5; targeted mutation 2, Frederick J de Sauvage
MGI ID: MGI:5294798
Synonyms: Lgr5DTR, Lgr5DTR-EGFP, Lgr5-DTR-GFP, Lgr5-EGFP-DTR, Lgr5tm2(DTR/EGFP)Fjs
Gene: Lgr5  Location: Chr10:115286219-115423685 bp, - strand  Genetic Position: Chr10, 63.51 cM
Alliance: Lgr5tm2(Hbegf/EGFP)Fjs page
Mutation
origin
Germline Transmission:  Earliest citation of germline transmission: J:177145
Parent Cell Line:  C2 (Nagy) (ES Cell)
Strain of Origin:  C57BL/6NTac
Mutation
description
Allele Type:    Targeted (Inserted expressed sequence, Null/knockout, Reporter)
Mutations:    Insertion, Intragenic deletion
 
Lgr5tm2(Hbegf/EGFP)Fjs expresses 1 gene
 
Mutation detailsA targeting vector was generated so that a DTR-EGFP-pA-loxP-neo-loxP cassette (diphtheria toxin (DT) cDNA linked in-frame to EGFP polyA-floxed neo) was inserted immediately after the ATG codon in exon 1 of the Lgr5 locus (deleting the remainder of exon 1 and splice donor site in intron 1 - a 212 bp deletion) by homologous recombination in C2 ES cells. Correctly targeted clones were transfected with an cre plasmid to remove the neo cassette. (J:177145)
Phenotypes
Loading...
View phenotypes and curated references for all genotypes (concatenated display).
Expression
In Mice Carrying this Mutation: 2 RNA-Seq or microarray experiment(s)
Find Mice (IMSR)
Mouse strains and cell lines available from the International Mouse Strain Resource (IMSR)
Carrying this Mutation:  Mouse Strains: 1 strain available      Cell Lines: 0 lines available
Carrying any Lgr5 Mutation:  58 strains or lines available
References
Original:  J:177145 Tian H, et al., A reserve stem cell population in small intestine renders Lgr5-positive cells dispensable. Nature. 2011 Oct 13;478(7368):255-9
All:  52 reference(s)

Contributing Projects:
Mouse Genome Database (MGD), Gene Expression Database (GXD), Mouse Models of Human Cancer database (MMHCdb) (formerly Mouse Tumor Biology (MTB)), Gene Ontology (GO)
Citing These Resources
Funding Information
Warranty Disclaimer, Privacy Notice, Licensing, & Copyright
Send questions and comments to User Support.
last database update
10/29/2024
MGI 6.24
The Jackson Laboratory