Tg(Bmi1-cre/ERT2)1Fjs
Transgene Detail

Summary

• Symbol: Tg(Bmi1-cre/ERT2)1Fjs
• Name: transgene insertion 1, Frederick J de Sauvage
• MGI ID: MGI:5294806
• Synonyms: Bmi1CreER
• Transgene: Tg(Bmi1-cre/ERT2)1Fjs Location: unknown
• Alliance: Tg(Bmi1-cre/ERT2)1Fjs page

Transgene origin

• Strain of Origin: C57BL/6

Transgene description

• Transgene Type: Transgenic (Inducible, Recombinase)
• Inducer: tamoxifen
• Mutation: Insertion
• Tg(Bmi1-cre/ERT2)1Fjs expression driven by 1 gene
  Transgene expression driven by:
  

  Organism	Driver Gene	Note
  mouse	Bmi1 (MGI:88174)

• Mutation details: A BAC (RP23-181D14) containing the Bmi1 locus was modified via recombineering methods by insertion of an IRES-cre/ERT2-pA-Frt-neo-Frt cassette at 85 base pairs 3' of the stop codon of Bmi1. The neo cassette was deleted by transforming the modified BAC into arabinose-induced cells expressing Flp. The final vector was injected into C57BL/6 blastocysts to generate germline-transmitting mice. (J:177145)

Recombinase activity

Activity:	Tissue activity of this recombinase allele
Driver:	Bmi1 (mouse) Summary of all recombinase alleles driven by Bmi1. Alliance mouse Bmi1 gene page

Find Mice (IMSR)

• Mouse strains and cell lines available from the International Mouse Strain Resource (IMSR)
• Carrying this Mutation: Mouse Strains: 0 strains available Cell Lines: 0 lines available

References

• Original: J:177145 Tian H, et al., A reserve stem cell population in small intestine renders Lgr5-positive cells dispensable. Nature. 2011 Oct 13;478(7368):255-9
• All: 1 reference(s)