S1pr1^tm1.1Cys
Targeted Allele Detail

Summary

• Symbol: S1pr1^tm1.1Cys
• Name: sphingosine-1-phosphate receptor 1; targeted mutation 1.1, Jason G Cyster
• MGI ID: MGI:5295051
• Synonyms: S1PR1^TSS
• Gene: S1pr1 Location: Chr3:115504082-115508704 bp, - strand Genetic Position: Chr3, 50.0 cM
• Alliance: S1pr1^tm1.1Cys page

Mutation origin

• Germline Transmission: Earliest citation of germline transmission: J:176118
• Parent Cell Line: E14 (ES Cell)
• Strain of Origin: 129P2/OlaHsd

Mutation description

• Allele Type: Targeted
• Mutations: Insertion, Nucleotide substitutions
• Mutation details: Nucleotide substitutions replaced the threonine and serines in the TxxSS motif in the last 12 amino acids of the sequence with alanines (p.T371A and p.(S374_S375delinsAA)). A floxP site flanked neomycin resistance gene cassette was inserted into the 3' UTR. Subsequent cre-mediated recombination removed the neo cassette. (J:176118)

Phenotypes

Key:

hm	homozygous	ht	heterozygous	tg	involves transgenes	√	phenotype observed
cn	conditional genotype	cx	complex: > 1 genome feature	ot	other: hemizygous, indeterminate,...	N	normal phenotype

Genotype/
Background:

	Allelic Composition	Genetic Background	Cell Line(s)
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hm1	S1pr1^tm1.1Cys/S1pr1^tm1.1Cys	involves: 129P2/OlaHsd * C3H * C57BL/6

Phenotypes:

Affected Systems	hm1
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hematopoietic system	√
decreased B cell number	√
abnormal B cell physiology	√
immune system	√
immune system phenotype	N
decreased B cell number	√
abnormal B cell physiology	√

View phenotypes and curated references for all genotypes (concatenated display).

Find Mice (IMSR)

• Mouse strains and cell lines available from the International Mouse Strain Resource (IMSR)
• Carrying this Mutation: Mouse Strains: 1 strain available Cell Lines: 0 lines available
• Carrying any S1pr1 Mutation: 32 strains or lines available

References

• Original: J:176118 Arnon TI, et al., GRK2-dependent S1PR1 desensitization is required for lymphocytes to overcome their attraction to blood. Science. 2011 Sep 30;333(6051):1898-903
• All: 3 reference(s)