Usp2^tm1.1Ssw
Targeted Allele Detail

Summary

• Symbol: Usp2^tm1.1Ssw
• Name: ubiquitin specific peptidase 2; targeted mutation 1.1, Simon S Wing
• MGI ID: MGI:5295262
• Gene: Usp2 Location: Chr9:43978318-44006924 bp, + strand Genetic Position: Chr9, 24.53 cM, cytoband B
• Alliance: Usp2^tm1.1Ssw page

Mutation origin

• Germline Transmission: Earliest citation of germline transmission: J:215857
• Parent Cell Line: Not Specified (ES Cell)
• Strain of Origin: Not Specified

Mutation description

• Allele Type: Targeted (Null/knockout)
• Mutations: Insertion, Intragenic deletion
• Mutation details: An FRT flanked neomycin/kanamycin selection cassette and loxP site were inserted upstream of exon 4 and a loxP site was inserted downstream of exon 6 via homologous recombination. Cre mediated recombination deleted exons 4 - 6. RT-PCR and western blot analysis confirmed the absence of wild-type RNA and protein expression in testes and MEFs, respectively, from homozygous mice. RT-PCR analysis did detect expression of a small transcript containing exons 7 and 9 in testes from homozygous mice. (J:215857)

Expression

In Structures Affected by this Mutation:

3 anatomical structure(s)

Find Mice (IMSR)

• Mouse strains and cell lines available from the International Mouse Strain Resource (IMSR)
• Carrying this Mutation: Mouse Strains: 0 strains available Cell Lines: 0 lines available
• Carrying any Usp2 Mutation: 34 strains or lines available

References

• Original: J:215857 Yang Y, et al., Regulation of behavioral circadian rhythms and clock protein PER1 by the deubiquitinating enzyme USP2. Biol Open. 2012 Aug 15;1(8):789-801
• All: 4 reference(s)