Igh^tm5.1Fwa
Targeted Allele Detail

Summary

• Symbol: Igh^tm5.1Fwa
• Name: immunoglobulin heavy chain complex; targeted mutation 5.1, Frederick W Alt
• MGI ID: MGI:5295440
• Synonyms: IGCR1/CBE^-
• Gene: Igh Location: Chr12:113222388-115973574 bp, - strand Genetic Position: Chr12, Syntenic

Mutation origin

• Germline Transmission: Earliest citation of germline transmission: J:176649
• Parent Cell Line: TC (ES Cell)
• Strain of Origin: 129S6/SvEvTac

Mutation description

• Allele Type: Targeted
• Mutations: Insertion, Intragenic deletion, Nucleotide substitutions
• Mutation details: Site-directed mutagenesis introduced scrambled mutations of the 20 bp CTCF binding site 1 (CBE1) and 19 bp CBE2 sites within the intergenic control region 1 (IGCR1). Cre-mediated recombination removed the neo cassette inserted into the IGCR1. (J:176649)

Find Mice (IMSR)

• Mouse strains and cell lines available from the International Mouse Strain Resource (IMSR)
• Carrying this Mutation: Mouse Strains: 0 strains available Cell Lines: 0 lines available
• Carrying any Igh Mutation: 44 strains or lines available

References

• Original: J:176649 Guo C, et al., CTCF-binding elements mediate control of V(D)J recombination. Nature. 2011 Sep 22;477(7365):424-30
• All: 2 reference(s)