Ptpn11^tm1Ckq
Targeted Allele Detail

Summary

• Symbol: Ptpn11^tm1Ckq
• Name: protein tyrosine phosphatase, non-receptor type 11; targeted mutation 1, Cheng-Kui Qu
• MGI ID: MGI:5295460
• Synonyms: Ptpn11^E76Kneo
• Gene: Ptpn11 Location: Chr5:121268596-121329460 bp, - strand Genetic Position: Chr5, 61.72 cM, cytoband F
• Alliance: Ptpn11^tm1Ckq page

Embryonic lethality in Ptpn11^tm1Ckq/Ptpn11⁺ Hprt1^{tm1(CAG-cre)Mnn}/Hprt1⁺ mice

Show the 2 phenotype image(s) involving this allele.

Mutation origin

• Germline Transmission: Earliest citation of germline transmission: J:177285
• Parent Cell Line: D1 (ES Cell)
• Strain of Origin: (129S6/SvEvTac x C57BL/6J)F1

Mutation description

Allele Type: Targeted (Conditional ready, Humanized sequence) Mutations: Insertion, Single point mutation Mutation details: Exon 3 was replaced with a floxed neo/STOP cassette and a modified exon 3 in which nucleotide substitution (GAA to AAA) results in the amino acid substitution of lysine for glutamic acid at position 76 (E76K), mimicking a mutation found in some childhood acute leukemia and juvenile myelomonocytic leukemia (JMML) patients. This allele remains inactive until deletion of the neo cassette through cre-mediated recombination. (J:177285)

Generation of the Ptpn11^tm1Ckq allele

Expression

In Structures Affected by this Mutation:

3 anatomical structure(s)

Find Mice (IMSR)

• Mouse strains and cell lines available from the International Mouse Strain Resource (IMSR)
• Carrying this Mutation: Mouse Strains: 0 strains available Cell Lines: 0 lines available
• Carrying any Ptpn11 Mutation: 46 strains or lines available

References

• Original: J:177285 Xu D, et al., Non-lineage/stage-restricted effects of a gain-of-function mutation in tyrosine phosphatase Ptpn11 (Shp2) on malignant transformation of hematopoietic cells. J Exp Med. 2011 Sep 26;208(10):1977-88
• All: 15 reference(s)