Dlx1^{tm1(cre/ERT2)Zjh}
Targeted Allele Detail

Summary

• Symbol: Dlx1^{tm1(cre/ERT2)Zjh}
• Name: distal-less homeobox 1; transgene insertion 1, Z Josh Huang
• MGI ID: MGI:5295937
• Synonyms: Dlx1-CreER, Dlx1-CreER-KI, Dlx1-CreERT2, Dlx1-CreERT2-KI
• Gene: Dlx1 Location: Chr2:71358457-71364325 bp, + strand Genetic Position: Chr2, 42.61 cM
• Alliance: Dlx1^{tm1(cre/ERT2)Zjh} page

Mutation origin

• Germline Transmission: Earliest citation of germline transmission: J:151755
• Parent Cell Line: v6.5 (ES Cell)
• Strain of Origin: (C57BL/6 x 129S4/SvJae)F1
• Project Collection: Neuroscience Blueprint cre

Mutation description

• Allele Type: Targeted (Inducible, Recombinase)
• Inducer: tamoxifen
• Mutation: Insertion
• Dlx1^{tm1(cre/ERT2)Zjh} expression driven by 1 gene
  Knock-in expression driven by:
  

  Organism	Driver Gene	Note
  mouse	Dlx1 (MGI:94901)

• Mutation details: A targeting vector was designed to insert a CreERT2 fusion gene, an SV40 polyA signal, and an frt-flanked neo cassette into the initiation codon of the distal-less homeobox 1 locus (Dlx1). The construct was electroporated into (C57BL/6 x 129S4Sv/Jae)-derived V6.5 embryonic stem (ES) cells. Correctly targeted ES cells were injected into recipient blastocysts and chimeric mice were bred with C57BL/6 mice to originate the colony. The mutation is predicted to result in a null allele. Tamoxifen-inducible Cre recombinase activity recapitulates the endogenous Dlx1 expression pattern, Following tamoxifen administration, Cre recombinase activity is observed in Dlx1 positive cortical GABAergic neurons, striatal neurons, and olfactory neurons. (J:151755)

Recombinase activity

Activity:	Tissue activity of this recombinase allele
Driver:	Dlx1 (mouse) Summary of all recombinase alleles driven by Dlx1. Comparative matrix view of recombinase activities Alliance mouse Dlx1 gene page

Find Mice (IMSR)

• Mouse strains and cell lines available from the International Mouse Strain Resource (IMSR)
• Carrying this Mutation: Mouse Strains: 1 strain available Cell Lines: 0 lines available
• Carrying any Dlx1 Mutation: 21 strains or lines available

References

• Original: J:151755 NIH Neuroscience Blueprint Cre Driver Network, Cre recombinase-expressing mice generated for the NIH Neuroscience Blueprint Cre Driver Network. MGI Direct Data Submission. 2009
• All: 14 reference(s)