Sox2^tm3(TK)Hoch
Targeted Allele Detail

Summary

• Symbol: Sox2^tm3(TK)Hoch
• Name: SRY (sex determining region Y)-box 2; targeted mutation 2, Konrad Hochedlinger
• MGI ID: MGI:5296002
• Synonyms: Sox2^TK
• Gene: Sox2 Location: Chr3:34704554-34706610 bp, + strand Genetic Position: Chr3, 16.93 cM, cytoband A2-B
• Alliance: Sox2^tm3(TK)Hoch page

Mutation origin

• Germline Transmission: Earliest citation of germline transmission: J:177655
• Parent Cell Line: v6.5 (ES Cell)
• Strain of Origin: (C57BL/6 x 129S4/SvJae)F1

Mutation description

• Allele Type: Targeted (Inserted expressed sequence)
• Mutation: Insertion
• Mutation details: A puromycin-deltaTK (truncated thymidine kinase) sequence was inserted into the Sox2 locus by homologous recombination in V6.5 ES cells, placing TK under control of endogenous regulatory elements. In the mouse line generated, exposure to gancyclovir (GCV) will result in death of Sox2-positive (TK-expressing) dividing cells. (J:177655)

Find Mice (IMSR)

• Mouse strains and cell lines available from the International Mouse Strain Resource (IMSR)
• Carrying this Mutation: Mouse Strains: 1 strain available Cell Lines: 0 lines available
• Carrying any Sox2 Mutation: 56 strains or lines available

References

• Original: J:177655 Arnold K, et al., Sox2(+) adult stem and progenitor cells are important for tissue regeneration and survival of mice. Cell Stem Cell. 2011 Oct 4;9(4):317-29
• All: 2 reference(s)