Nudc^{tm1a(KOMP)Mbp}
Targeted Allele Detail

Summary

• Symbol: Nudc^{tm1a(KOMP)Mbp}
• Name: nudC nuclear distribution protein; targeted mutation 1a, Mouse Biology Program, UC Davis
• MGI ID: MGI:5296095
• Synonyms: NudC^GT
• Gene: Nudc Location: Chr4:133259853-133273307 bp, - strand Genetic Position: Chr4, 66.25 cM, cytoband D3
• Alliance: Nudc^{tm1a(KOMP)Mbp} page
• IMPC: Nudc gene page

Mutation origin

• Mutant Cell Lines: DEPD00577_3_A11, DEPD00577_3_B09, DEPD00577_3_D12, DEPD00577_3_F09, DEPD00577_3_F10, DEPD00577_3_H09, DEPD00577_3_H11
• Germline Transmission: Earliest citation of germline transmission: J:346299
• Parent Cell Line: JM8A3.N1 (ES Cell)
• Strain of Origin: C57BL/6N-A^tm1Brd
• Project Collection: KOMP-CSD

Mutation description

• Allele Type: Targeted (Conditional ready, Reporter)
• Mutation: Insertion Vector: L1L2_Bact_P
• Mutation details: The L1L2_Bact_P cassette was inserted at position 133260405 of Chromosome 4 upstream of the critical exons 3-7 (Build GRCm39). The cassette is composed of an FRT site followed by lacZ sequence and a loxP site. This first loxP site is followed by a neomycin resistance gene under the control of the human beta-actin promoter, SV40 polyA, a second FRT site and a second loxP site. A third loxP site is inserted downstream of the targeted exons 3-7 at position 133263632. The critical exons 3 to 7 are thus flanked by loxP sites. A "conditional ready" (floxed) allele can be created by flp recombinase expression in mice carrying this allele. Subsequent cre expression results in a knockout mouse. If cre expression occurs without flp expression, a reporter knockout mouse will be created. Further information on targeting strategies used for this and other IKMC alleles can be found at http://www.informatics.jax.org/mgihome/nomen/IKMC_schematics.shtml. Homozygous mice do not show a phenotype indicating a leaky gene trap and that this is not a knock-out allele. (J:157064, J:346299)

Find Mice (IMSR)

• Mouse strains and cell lines available from the International Mouse Strain Resource (IMSR)
• Carrying this Mutation: Mouse Strains: 0 strains available Cell Lines: 1 line available
• Carrying any Nudc Mutation: 21 strains or lines available

References

• Original: J:157064 University of California, Davis, Alleles produced for the KOMP project by the University of California, Davis. MGI Direct Data Submission. 2010
• All: 2 reference(s)