Fn1^tm2.1Ref
Targeted Allele Detail

Summary

• Symbol: Fn1^tm2.1Ref
• Name: fibronectin 1; targeted mutation 2.1, Reinhard Fassler
• MGI ID: MGI:5296500
• Synonyms: FN^RGE
• Gene: Fn1 Location: Chr1:71624679-71692359 bp, - strand Genetic Position: Chr1, 36.05 cM, cytoband C1-C5
• Alliance: Fn1^tm2.1Ref page

Mutation origin

• Germline Transmission: Earliest citation of germline transmission: J:177821
• Parent Cell Line: R1 (ES Cell)
• Strain of Origin: (129X1/SvJ x 129S1/Sv)F1-Kitl⁺

Mutation description

• Allele Type: Targeted (Null/knockout)
• Mutation: Single point mutation
• Mutation details: A C1238G mutation in exon 30 results in an aspartic to glutamic acid change altering the RGD motif to RGE. A floxed neomycin resistance cassette was inserted in intron 30 and subsequently removed from properly targeted mice by cre excision. (J:177821)

Expression

In Structures Affected by this Mutation:

5 anatomical structure(s)

Find Mice (IMSR)

• Mouse strains and cell lines available from the International Mouse Strain Resource (IMSR)
• Carrying this Mutation: Mouse Strains: 0 strains available Cell Lines: 0 lines available
• Carrying any Fn1 Mutation: 130 strains or lines available

References

• Original: J:177821 Takahashi S, et al., The RGD motif in fibronectin is essential for development but dispensable for fibril assembly. J Cell Biol. 2007 Jul 2;178(1):167-78
• All: 6 reference(s)