Eomes^{tm3.1(cre)Rob}
Targeted Allele Detail

Summary

• Symbol: Eomes^{tm3.1(cre)Rob}
• Name: eomesodermin; targeted mutation 3.1, Elizabeth J Robertson
• MGI ID: MGI:5296755
• Synonyms: Eomes^Cre
• Gene: Eomes Location: Chr9:118307259-118315176 bp, + strand Genetic Position: Chr9, 70.21 cM, cytoband F3
• Alliance: Eomes^{tm3.1(cre)Rob} page

Mutation origin

• Germline Transmission: Earliest citation of germline transmission: J:176969
• Parent Cell Line: CCE/EK.CCE (ES Cell)
• Strain of Origin: 129S/SvEv-Gpi1^c

Mutation description

• Allele Type: Targeted (Recombinase)
• Mutations: Insertion, Intragenic deletion
• Eomes^{tm3.1(cre)Rob} expression driven by 1 gene
  Knock-in expression driven by:
  

  Organism	Driver Gene	Note
  mouse	Eomes (MGI:1201683)

• Mutation details: A targeting vector introduced the coding sequence for cre recombinase, followed by a loxP-flanked neomycin selection cassette was introduced into the exon 1 start site of the Eomes gene, resulting in deletion of about 500 base pairs of the 5' coding region. Targeting was done in CCE ES cells. Correctly targeted ES cells were transiently transfected with a plasmed encoding cre to remove the neomycing cassette. The resulting cells were used to generate germline chimeras. Cre mRNA expression recapitulated endogenous Eomes expression. (J:176969)

Recombinase activity

Activity:	Tissue activity of this recombinase allele
Driver:	Eomes (mouse) Summary of all recombinase alleles driven by Eomes. Alliance mouse Eomes gene page

Find Mice (IMSR)

• Mouse strains and cell lines available from the International Mouse Strain Resource (IMSR)
• Carrying this Mutation: Mouse Strains: 0 strains available Cell Lines: 0 lines available
• Carrying any Eomes Mutation: 44 strains or lines available

References

• Original: J:176969 Costello I, et al., The T-box transcription factor Eomesodermin acts upstream of Mesp1 to specify cardiac mesoderm during mouse gastrulation. Nat Cell Biol. 2011 Sep;13(9):1084-91
• All: 6 reference(s)