Napepld^tm1.2Ueda
Targeted Allele Detail

Summary

• Symbol: Napepld^tm1.2Ueda
• Name: N-acyl phosphatidylethanolamine phospholipase D; targeted mutation 1.2, Natsuo Ueda
• MGI ID: MGI:5296775
• Synonyms: NAPE-PLD^-, Napepld^tm1.2Itl
• Gene: Napepld Location: Chr5:21867899-21906394 bp, - strand Genetic Position: Chr5, 9.97 cM
• Alliance: Napepld^tm1.2Ueda page

Mutation origin

• Germline Transmission: Earliest citation of germline transmission: J:177598
• Parent Cell Line: iTL1 (ES Cell)
• Strain of Origin: 129S6/SvEvTac

Mutation description

• Allele Type: Targeted (Null/knockout)
• Mutations: Insertion, Intragenic deletion
• Mutation details: A loxP site was inserted upstream of exon 3. An FRT-flanked neo cassette with a 3' loxP site was inserted downstream of exon 3. Flp-mediated recombination removed the neo cassette. Cre-mediated recombination removed exon 3. The absence of protein expression was confirmed by western blot analysis on brain extracts. (J:177598)

Find Mice (IMSR)

• Mouse strains and cell lines available from the International Mouse Strain Resource (IMSR)
• Carrying this Mutation: Mouse Strains: 0 strains available Cell Lines: 0 lines available
• Carrying any Napepld Mutation: 25 strains or lines available

References

• Original: J:177598 Tsuboi K, et al., Enzymatic formation of N-acylethanolamines from N-acylethanolamine plasmalogen through N-acylphosphatidylethanolamine-hydrolyzing phospholipase D-dependent and -independent pathways. Biochim Biophys Acta. 2011 Oct;1811(10):565-77
• All: 2 reference(s)