Itgb1^tm10.1Ref
Targeted Allele Detail

Summary

• Symbol: Itgb1^tm10.1Ref
• Name: integrin beta 1 (fibronectin receptor beta); targeted mutation 10.1, Reinhard Fassler
• MGI ID: MGI:5296845
• Synonyms: Y795F
• Gene: Itgb1 Location: Chr8:129412135-129459681 bp, + strand Genetic Position: Chr8, 76.09 cM, cytoband E2
• Alliance: Itgb1^tm10.1Ref page

Mutation origin

• Germline Transmission: Earliest citation of germline transmission: J:177979
• Parent Cell Line: R1 (ES Cell)
• Strain of Origin: (129X1/SvJ x 129S1/Sv)F1-Kitl⁺

Mutation description

Allele Type: Targeted (Not Specified) Mutations: Insertion, Single point mutation Mutation details: A floxed neo cassette was inserted downstream of a modified exon 16 in which nucleotide substitution results in the amino acid substitution of phenylalanine for tyrosine at position 795 (Y795F). Cre-mediated recombination removed the neo cassette. (J:177979)

Targeting strategy for introducing point mutations in the cytoplasmic domain of Itgb1

Expression

In Structures Affected by this Mutation:

1 anatomical structure(s)

Find Mice (IMSR)

• Mouse strains and cell lines available from the International Mouse Strain Resource (IMSR)
• Carrying this Mutation: Mouse Strains: 0 strains available Cell Lines: 0 lines available
• Carrying any Itgb1 Mutation: 60 strains or lines available

References

• Original: J:177979 Czuchra A, et al., Genetic analysis of beta1 integrin "activation motifs" in mice. J Cell Biol. 2006 Sep 11;174(6):889-99
• All: 3 reference(s)