Mirc12^tm1.1Doca
Targeted Allele Detail

Summary

• Symbol: Mirc12^tm1.1Doca
• Name: microRNA cluster 12, including Mir144 through Mir451a; targeted mutation 1.1, Donal O'Carroll
• MGI ID: MGI:5296851
• Synonyms: miR144/451^eGFP
• Gene: Mirc12 Location: Chr11:77963831-77964067 bp Genetic Position: Chr11, Syntenic

Mutation origin

• Germline Transmission: Earliest citation of germline transmission: J:177074
• Parent Cell Line: A9 (ES Cell)
• Strain of Origin: (C57BL/6 x 129P2/OlaHsd)F1

Mutation description

• Allele Type: Targeted (Null/knockout, Reporter)
• Mutation: Insertion
• Mutation details: An EGFP expression cassette as well as an FRT flanked neomycin resistance gene was inserted into the Mir144 hairpin. The EGFP construct includes a synthetic intronic sequence and the strong late polyadenylation signal from SV40. The selection gene was excised by crossing to flp deleter mice. Insertion of the EGFP reporter in Mir144 disrupts expression of both Mir144 and Mir451 which are transcribed as a single precursor RNA. (J:177074)

Find Mice (IMSR)

• Mouse strains and cell lines available from the International Mouse Strain Resource (IMSR)
• Carrying this Mutation: Mouse Strains: 1 strain available Cell Lines: 0 lines available
• Carrying any Mirc12 Mutation: 2 strains or lines available

References

• Original: J:177074 Rasmussen KD, et al., The miR-144/451eGFP allele, a novel tool for resolving the erythroid potential of hematopoietic precursors. Blood. 2011 Sep 15;118(11):2988-92
• All: 3 reference(s)