Bhmt^tm1.2Zei
Targeted Allele Detail

Summary

• Symbol: Bhmt^tm1.2Zei
• Name: betaine-homocysteine methyltransferase; targeted mutation 1.2, Steven Zeisel
• MGI ID: MGI:5297083
• Gene: Bhmt Location: Chr13:93753399-93774266 bp, - strand Genetic Position: Chr13, 47.87 cM, cytoband D1
• Alliance: Bhmt^tm1.2Zei page

Mutation origin

• Germline Transmission: Earliest citation of germline transmission: J:177569
• Parent Cell Line: E14TG2a (ES Cell)
• Strain of Origin: 129P2/OlaHsd

Mutation description

• Allele Type: Targeted (Null/knockout)
• Mutations: Insertion, Intragenic deletion
• Mutation details: One loxAPA site was inserted in intron 5 240 bp upstream of exon 6 and an FRT flanked MC1-neomycin cassette followed by a second loxP site was placed 330 bp from exon 7. The neomycin selection cassette was excised by crossing to Tg(ACTFLPe)9205Dym mice. Exons 6 and 7, containing the essential zinc binding domain, were then removed by crossing to Tg(EIIa-cre)C5379Lmgd mice. (J:177569)

Expression

In Mice Carrying this Mutation:	18 assay results 1 RNA-Seq or microarray experiment(s)
In Structures Affected by this Mutation:	3 anatomical structure(s)

Find Mice (IMSR)

• Mouse strains and cell lines available from the International Mouse Strain Resource (IMSR)
• Carrying this Mutation: Mouse Strains: 1 strain available Cell Lines: 0 lines available
• Carrying any Bhmt Mutation: 23 strains or lines available

References

• Original: J:177569 Teng YW, et al., Deletion of betaine-homocysteine s-methyltransferase in mice perturbs choline and 1-carbon metabolism, resulting in Fatty liver and hepatocellular carcinomas. J Biol Chem. 2011 Oct 21;286(42):36258-67
• All: 5 reference(s)