About   Help   FAQ
Gt(ROSA)26Sortm3(tetO-EGFP,-RNAi:T)Bgh
Targeted Allele Detail
Summary
Symbol: Gt(ROSA)26Sortm3(tetO-EGFP,-RNAi:T)Bgh
Name: gene trap ROSA 26, Philippe Soriano; targeted mutation 3, Bernhard G Herrmann
MGI ID: MGI:5297100
Synonyms: KD3-T
Gene: Gt(ROSA)26Sor  Location: Chr6:113044389-113054205 bp, - strand  Genetic Position: Chr6, 52.73 cM
Alliance: Gt(ROSA)26Sortm3(tetO-EGFP,-RNAi:T)Bgh page
Mutation
origin
Germline Transmission:  Earliest citation of germline transmission: J:190628
Parent Cell Line:  Other (see notes) (ES Cell)
Strain of Origin:  Not Specified
Mutation
description
Allele Type:    Targeted (Inducible, Knockdown, RMCE-ready)
Inducer:    doxycycline/tetracycline
Mutation:    Insertion
  Gt(ROSA)26Sortm3(tetO-EGFP,-RNAi:T)Bgh involves 1 genes/genome features (T) View all
 
Mutation detailsRecombinase mediated cassette exchange was used to insert a tetO, CAG regulated EGFP followed by a short hairpin RNAi specific for the Brachyury (T) gene followed by a PGK promoter into the Gt(ROSA)26Sortm1Bgh allele. The tetracycline silencer prevents expression of both the reporter and the shRNAi until mice are treated with doxycycline. (J:173434)
Phenotypes
Loading...
View phenotypes and curated references for all genotypes (concatenated display).
Find Mice (IMSR)
Mouse strains and cell lines available from the International Mouse Strain Resource (IMSR)
Carrying this Mutation:  Mouse Strains: 0 strains available      Cell Lines: 0 lines available
Carrying any Gt(ROSA)26Sor Mutation:  991 strains or lines available
Notes
ES cell lines = C57BL/6 and G4.
References
Original:  J:173434 Vidigal JA, et al., An inducible RNA interference system for the functional dissection of mouse embryogenesis. Nucleic Acids Res. 2010 Jun 1;38(11):e122
All:  2 reference(s)

Contributing Projects:
Mouse Genome Database (MGD), Gene Expression Database (GXD), Mouse Models of Human Cancer database (MMHCdb) (formerly Mouse Tumor Biology (MTB)), Gene Ontology (GO)
Citing These Resources
Funding Information
Warranty Disclaimer, Privacy Notice, Licensing, & Copyright
Send questions and comments to User Support.
last database update
10/22/2024
MGI 6.24
The Jackson Laboratory