Gt(ROSA)26Sortm3(tetO-EGFP,-RNAi:T)Bgh
Targeted Allele Detail
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Symbol: |
Gt(ROSA)26Sortm3(tetO-EGFP,-RNAi:T)Bgh |
Name: |
gene trap ROSA 26, Philippe Soriano; targeted mutation 3, Bernhard G Herrmann |
MGI ID: |
MGI:5297100 |
Synonyms: |
KD3-T |
Gene: |
Gt(ROSA)26Sor Location: Chr6:113044389-113054205 bp, - strand Genetic Position: Chr6, 52.73 cM
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Alliance: |
Gt(ROSA)26Sortm3(tetO-EGFP,-RNAi:T)Bgh page
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Germline Transmission: |
Earliest citation of germline transmission:
J:190628
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Parent Cell Line: |
Other (see notes) (ES Cell)
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Strain of Origin: |
Not Specified
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Allele Type: |
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Targeted (Inducible, Knockdown, RMCE-ready) |
Inducer: |
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doxycycline/tetracycline |
Mutation: |
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Insertion
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Gt(ROSA)26Sortm3(tetO-EGFP,-RNAi:T)Bgh involves 1 genes/genome features (T)
View all
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Mutation details: Recombinase mediated cassette exchange was used to insert a tetO, CAG regulated EGFP followed by a short hairpin RNAi specific for the Brachyury (T) gene followed by a PGK promoter into the Gt(ROSA)26Sortm1Bgh allele. The tetracycline silencer prevents expression of both the reporter and the shRNAi until mice are treated with doxycycline.
(J:173434)
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View phenotypes and curated references for all genotypes (concatenated display).
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Mouse strains and cell lines
available from the International Mouse Strain Resource
(IMSR) |
Carrying this Mutation: |
Mouse Strains: 0 strains available
Cell Lines: 0 lines available
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Carrying any Gt(ROSA)26Sor Mutation: |
993 strains or lines available
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ES cell lines = C57BL/6 and G4.
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Original: |
J:173434 Vidigal JA, et al., An inducible RNA interference system for the functional dissection of mouse embryogenesis. Nucleic Acids Res. 2010 Jun 1;38(11):e122 |
All: |
2 reference(s) |
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